論文

1997年

A method of primary cell culture for establishing equine long-term culture cell lines

Journal of Equine Science
  • Seiki Watanabe
  • ,
  • Youichirou Ishikawa
  • ,
  • Hiromi Hara
  • ,
  • Kei Hanzawa
  • ,
  • Harutaka Mukoyama

8
4
開始ページ
95
終了ページ
99
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1294/jes.8.95
出版者・発行元
Japanese Society of Equine Science

We investigated a method of culture for the establishment of equine long-term culture cell lines. The tissues tested were equine fetus tissues
right abdomen epithelium, the umbilical artery, the umbilical ring, the pulmonary artery, the renal cortex and the fetal placenta. To decide on a suitable primary culture method, we compared two methods of primary culture: tissue fragment culture and low-temperature trypsin cell-dispersal. On the other hand, to decide on a suitable culture medium, we compared 11 culture media: BME, MEM, D-MEM, IMDM and HFM (commercially completed culture media, available Human Foreskin Melanocyte). These were supplemented with 10% or 20% FCS when used as culture media. Moreover, HFM medium was used with no FCS, 10% and 20% FCS. Culture conditions were 37°C, 700% humidity, 5% CO2 and 55% air. We obtained the following results: 1) The tissue fragment culture method was suitable for preparing primary cell culture from fetal tissue. 2) High-nutrition culture media such as D-MEM and IMDM were suitable for the culture of fetal tissues. 3) We established a cell line from cells of renal cortex, named "TEK-1 ".

リンク情報
DOI
https://doi.org/10.1294/jes.8.95
ID情報
  • DOI : 10.1294/jes.8.95
  • ISSN : 1347-7501
  • ISSN : 1340-3516
  • SCOPUS ID : 53249100607

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