論文

査読有り
2010年1月

Cloning and characterization of grpE in Acetobacter pasteurianus NBRC 3283

JOURNAL OF BIOSCIENCE AND BIOENGINEERING
  • Morio Ishikawa
  • ,
  • Akiko Okamoto-Kainuma
  • ,
  • Takayuki Jochi
  • ,
  • Ikue Suzuki
  • ,
  • Kazuaki Matsui
  • ,
  • Takayuki Kaga
  • ,
  • Yukimichi Koizumi

109
1
開始ページ
25
終了ページ
31
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jbiosc.2009.07.008
出版者・発行元
SOC BIOSCIENCE BIOENGINEERING JAPAN

The grpE gene in Acetobacter pasteurianus NBRC 3283 was cloned and characterized, to elucidate the mechanism underlying the resistance of acetic acid bacteria to the stressors existing during acetic acid fermentation. This gene was found to be located in tandem with two related genes, appearing on the genome in the order grpE-dnaK-dnaJ. A sigma(32)-type promoter sequence was found in the upstream region of grpE. The relative transcription levels of grpE, dnaK, and dnaJ mRNA were in the ratio of approximately 1:2:0.1, and the genes were transcribed as grpE-dnaK, dnaK, and dnaJ. The transcription level of grpE was elevated by heat shock and treatment with ethanol. Co-overexpression of GrpE with DnaK/J in cells resulted in improved growth compared to the single overexpression of DnaK/J in high temperature or ethanol-containing conditions, suggesting that GrpE acts cooperatively with DnaK/J for expressing resistance to those stressors considered to exist during acetic acid fermentation. Our findings indicate that GrpE is closely associated with adaptation to stressors in A. pasteurianus and may play an important role in acetic acid fermentation. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.jbiosc.2009.07.008
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000274765000005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.jbiosc.2009.07.008
  • ISSN : 1389-1723
  • Web of Science ID : WOS:000274765000005

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