論文

査読有り
2011年4月

Cell proliferation in visceral organs induced by ventromedial hypothalamic (VMH) lesions: Development of electrical VMH lesions in mice and resulting pathophysiological profiles

ENDOCRINE JOURNAL
  • Yoko Suzuki
  • Shuji Inoue
  • Hiroyuki Shimizu
  • Noriko Ishizuka
  • Yoshiko Kasahara
  • Tosei Takahashi
  • Katsumi Arai
  • Yoko Kobayashi
  • Mikiko Kishi
  • Nobuo Imazeki
  • Akira Senoo
  • Toshimasa Osaka
  • 全て表示

58
4
開始ページ
247
終了ページ
256
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1507/endocrj.K10E-408
出版者・発行元
JAPAN ENDOCRINE SOC

We have found that ventromedial hypothalamic (VMH) lesions produced by electrocoagulation induce cell proliferation in visceral organs through vagal hyperactivity, and also stimulate regeneration of partially resected liver in rats. To facilitate identification of proliferative and/or regenerative factors at the gene level, we developed electrical production of VMH lesions in mice, for which more genetic information is available compared to rats, and examined the pathophysiological profiles in these mice. Using ddy mice, we produced VMH lesions with reference to the previously reported method in rats. We then examined the pathophysiological profiles of the VMH-lesioned mice. Electrical VMH lesions in mice were produced using the following coordinates: 1.6 mm posterior to the bregma, anteriorly; 0.5 mm lateral to the midsagittal line, transversely; and 0.2 mm above the base of the skull, vertically, with 1 mA of current intensity and 10 s duration. The VMH-lesioned mice showed similar metabolic characteristics to those of VMH-lesioned rats, including body weight gain, increased food intake, increased percentage body fat, and elevated serum insulin and leptin. However, there were some differences in short period of hyperphagia, and in normal serum lipids compared to those of VMH-lesioned rats. The mice showed a similar cell proliferation in visceral organs, including stomach, small intestine, liver, and, exocrine and endocrine pancreas. In conclusion, procedures for development of VMH lesions in mice by elcctrocoagulation were developed and the VMH-lesioned mice showed pathophysiological profiles similar to those of VMH-lesioned rats, particularly in cell proliferation in visceral organs. These findings have not been observed previously in gold thioglucose-induced VMH-lesioned mice. This model may be a new tool for identifying factors involved in cell proliferation or regeneration in visceral organs.

リンク情報
DOI
https://doi.org/10.1507/endocrj.K10E-408
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21325743
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000290634400003&DestApp=WOS_CPL
ID情報
  • DOI : 10.1507/endocrj.K10E-408
  • ISSN : 0918-8959
  • PubMed ID : 21325743
  • Web of Science ID : WOS:000290634400003

エクスポート
BibTeX RIS