2011年
Full-length cDNA overexpressor gene hunting system (FOX hunting system).
Methods in molecular biology (Clifton, N.J.)
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- 巻
- 678
- 号
- 開始ページ
- 77
- 終了ページ
- 89
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1007/978-1-60761-682-5_7
Full-length cDNAs (fl-cDNAs) are important resources for the characterization of gene function, since they contain all the information required for the production of functional RNAs and proteins. Large sets of fl-cDNA clones have been collected from several plant species and have become available for functional genomic analysis. We have developed a system for the identification of gene function by screening for transgenic plants ectopically expressing fl-cDNAs and named it the FOX (fl-cDNA overexpressor gene) hunting system. This system can be applied to almost all plant species without prior knowledge of their genome sequences because only fl-cDNAs are required. For utilization of the FOX hunting system, Agrobacterium libraries and Arabidopsis seeds carrying rice and Arabidopsis fl-cDNAs are available. Here, we will describe the procedure followed in the FOX hunting system from the generation of expression vectors carrying fl-cDNAs to the confirmation of phenotype in retransformed plants.
- リンク情報
- ID情報
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- DOI : 10.1007/978-1-60761-682-5_7
- PubMed ID : 20931374