J-GLOBAL         Last updated: Nov 14, 2018 at 13:49
Kyushu University
Faculty of Agriculture
Job title
Dr. of Agriculture(Nagoya Univ.)

Research Areas


Academic & Professional Experience

Sep 1996
Sep 1998
Visiting Associate Research Biochemist, Department of Molecular and Cell Biology, University of California, Berkeley

Published Papers

Abiodun M.O, Matsuoka K.
Methods in Enzymology   588 515-526   2017   [Refereed]
Kaneko K, Takamatsu T, Inomata T, Oikawa K, Itoh K, Hirose K, Amano M, Nishimura S.-I, Toyooka K, Matsuoka K, Pozueta-Romero J, Mitsui T.
Plant and Cell Physiology   57(8) 1610-1628   2016   [Refereed]
Liu J, Hayashi K, Matsuoka K.
Bioscience, Biotechnology and Biochemistry   79(12) 2007-2013   2015   [Refereed]
Velasquez S.M, Ricardi M.M, Poulsen C.P, Oikawa A, Dilokpimol A, Halim A, Mangano S, Denita Juarez S.P, Marzol E, Salgado Salter J.D, Dorosz J.G, Borassi C, M{ö}ller S.R, Buono R, Ohsawa Y, Matsuoka K, Otegui M.S, Scheller H.V, Geshi N, Petersen B.L, Iusem N.D, Estevez J.M.
Molecular Plant   8(5) 734-746   2015   [Refereed]
The international conference on plant factory 2014
93) Toshihiko Eguchi, Hiroyuki Tanaka, Satoshi Yoshida, Ken Matsuoka
The international conference on plant factory 2014      Nov 2014   [Refereed]
Toyooka K, Sato M, Kutsuna N, Higaki T, Sawaki F, Wakazaki M, Goto Y, Hasezawa S, Nagata N, Matsuoka K.
Plant and Cell Physiology   55(9) 1544-1555   2014   [Refereed]
Saito F, Suyama A, Oka T, Yoko-O T, Matsuoka K, Jigami Y, Shimma Y.-I.
Journal of Biological Chemistry   289(30) 20405-20420   2014   [Refereed]
Noboru Yamauchi, Tadashi Gosho, Satoru Asatuma, Satoru Asatuma, Kiminori Toyooka, Kiminori Toyooka, Toru Fujiwara, Toru Fujiwara, Ken Matsuoka
F1000Research   2    Sep 2013
© 2013 Yamauchi N et al. In Arabidopsis the borate transporter BOR1, which is located in the plasma membrane, is degraded in the presence of excess boron by an endocytosis-mediated mechanism. A similar mechanism was suggested in rice as excess bor...
Moses Olabiyi Abiodun, Ken Matsuoka
Plant Signaling and Behavior   8    Aug 2013
We have recently developed a new method aimed at mass photo-conversion of photo-convertible fluorescence protein (PFP) fluorescence in transformed tobacco BY-2 cells. Using this method we reported recently that the Golgi apparatus is generated by ...
Koshiba T, Kobayashi M, Matsuoka K, Fujiwara T, Matoh T.
Soil Science and Plant Nutrition   59(2) 189-194   2013   [Refereed]
Naoko Munemasa, Akiko Suyama, Ken Matsuoka
Bioscience, Biotechnology and Biochemistry   76 2159-2161   Dec 2012
NtP4H1.1 is a Golgi-localizing type II integral membrane protein. Mutations in the cytoplasmic tail direct the protein to the endoplasmic reticulum (ER). We expressed a GFP fusion protein containing the mutant tail and the transmembrane region in ...
Hiromasa Shikata, Mami Shibata, Tomokazu Ushijima, Moeko Nakashima, Sam Geun Kong, Ken Matsuoka, Chentao Lin, Tomonao Matsushita
Plant Journal   70 727-738   Jun 2012
Plants monitor the light environment through informational photoreceptors that include phytochromes. In seedling de-etiolation, phytochrome B (phyB), which is the most important member of the phytochrome family, interacts with transcription factor...
Keiko Kobayashi, Keiko Kobayashi, Masuyo Kawabata, Masuyo Kawabata, Keizo Hisano, Tomohiko Kazama, Ken Matsuoka, Ken Matsuoka, Ken Matsuoka, Mamoru Sugita, Takahiro Nakamura, Takahiro Nakamura, Takahiro Nakamura, Takahiro Nakamura
Nucleic Acids Research   40 2712-2723   Mar 2012
The expressions of chloroplast and mitochondria genes are tightly controlled by numerous nuclear-encoded proteins, mainly at the post-transcriptional level. Recent analyses have identified a large, plant-specific family of pentatricopeptide repeat...
Hiromasa Shikata, Moeko Nakashima, Ken Matsuoka, Ken Matsuoka, Tomonao Matsushita
Plant Signaling and Behavior   7    Jan 2012
Phytochrome B (phyB), a major photoreceptor in plants, interacts with transcription factors to regulate gene expression and induce various light responses. Recently, we identified an SR-like splicing factor, RRC1 (reduced red-light responses in cr...
Masaru Kobayashi, Nagisa Kouzu, Akina Inami, Kiminori Toyooka, Yuki Konishi, Ken Matsuoka, Toru Matoh
Plant and Cell Physiology   52 1832-1843   Oct 2011
In plant cells, boron (B) occurs predominantly as a borate ester associated with rhamnogalacturonan II (RG-II), but the function of this B-RG-II complex has yet to be investigated. 3-Deoxy-d-manno-2-octulosonic acid (KDO) is a specific component m...
Masaro Akai, Kiyoshi Onai, Miyako Kusano, Mayuko Sato, Henning Redestig, Kiminori Toyooka, Megumi Morishita, Hiroshi Miyake, Akihiro Hazama, Vanessa Checchetto, Ildikò Szabò, Ken Matsuoka, Ken Matsuoka, Kazuki Saito, Kazuki Saito, Masato Yasui, Masahiro Ishiura, Nobuyuki Uozumi
Journal of Biological Chemistry   286 25224-25235   Jul 2011
The genome of Synechocystis PCC 6803 contains a single gene encoding an aquaporin, aqpZ. The AqpZ protein functioned as a water-permeable channel in the plasma membrane. However, the physiological importance of AqpZ in Synechocystis remains unclea...
Ryo Moriguchi, Chiaya Matsuoka, Akiko Suyama, Ken Matsuoka, Ken Matsuoka
Bioscience, Biotechnology and Biochemistry   75 994-996   Jun 2011
Plant specific O-glycosylation of proteins includes the attachment of arabinogalactan to hydroxyproline (Hyp) residues. These Hyp residues are generated from peptidyl proline residues by the action of prolyl 4-hydroxylase which requires the ferrou...
Mst Nasrin Akhter Banu, Md Anamul Hoque, Megumi Watanabe-Sugimoto, Mohammad Muzahidul Islam, Misugi Uraji, Ken Matsuoka, Yoshimasa Nakamura, Yoshiyuki Murata
Bioscience, Biotechnology and Biochemistry   74 2043-2049   Nov 2010
Efficient detoxification of the reactive oxygen species, nitric oxide (NO) and methylglyoxal (MG), provides protection against NaCl-induced damage in plants. To elucidate the protective mechanisms of proline and glycinebetaine (betaine) against Na...
Nawaporn Onkokesung, Ivan Gális, Caroline C. von Dahl, Caroline C. von Dahl, Ken Matsuoka, Hans Peter Saluz, Ian T. Baldwin
Plant Physiology   153 785-798   Jun 2010
Jasmonic acid (JA) and ethylene (ET) are known to play important roles in mediating plant defense against herbivores, but how they affect development in herbivore-attacked plants is unknown. We used JA-deficient (silenced in LIPOXYGENASE3 [asLOX3]...
Takayuki Sasaki, Izumi C. Mori, Takuya Furuichi, Shintaro Munemasa, Kiminori Toyooka, Ken Matsuoka, Yoshiyuki Murata, Yoko Yamamoto
Plant and Cell Physiology   51 354-365   Mar 2010
Plant stomata limit both carbon dioxide uptake and water loss; hence, stomatal aperture is carefully set as the environment fluctuates. Aperture area is known to be regulated in part by ion transport, but few of the transporters have been characte...
Takuji Oka, Takuji Oka, Fumie Saito, Fumie Saito, Yoh ichi Shimma, Yoh ichi Shimma, Takehiko Yoko-o, Takehiko Yoko-o, Yoshiyuki Nomura, Ken Matsuoka, Yoshifumi Jigami, Yoshifumi Jigami
Plant Physiology   152 332-340   Jan 2010
We characterized peptidyl hydroxyproline (Hyp) O-galactosyltransferase (HGT), which is the initial enzyme in the arabinogalactan biosynthetic pathway. An in vitro assay of HGT activity was established using chemically synthesized fluorescent pepti...
Aya Kitajima, Satoru Asatsuma, Satoru Asatsuma, Hisao Okada, Yuki Hamada, Kentaro Kaneko, Yohei Nanjo, Yohei Nanjo, Yasushi Kawagoe, Kiminori Toyooka, Ken Matsuoka, Ken Matsuoka, Masaki Takeuchi, Masaki Takeuchi, Akihiko Nakano, Akihiko Nakano, Toshiaki Mitsui
Plant Cell   21(9) 2844-2858   Dec 2009
The well-characterized secretory glycoprotein, rice [Oryza sativa) α-amylase isoform I-1 (Amyl-1), was localized within the plastids and proved to be involved in the degradation of starch granules in the organelles of rice cells. In addition, a la...
Kenta Tsunekawa, Toshiaki Shijuku, Mitsuo Hayashimoto, Yoichi Kojima, Kiyoshi Onai, Megumi Morishita, Masahiro Ishiura, Teruo Kuroda, Tatsunosuke Nakamura, Hiroshi Kobayashi, Mayuko Sato, Kiminori Toyooka, Ken Matsuoka, Tatsuo Omata, Nobuyuki Uozumi
Journal of Biological Chemistry   284 16513-16521   Jun 2009
Na+/H+antiporters influence proton or sodium motive force across the membrane. Synechocystis sp. PCC 6803 has six genes encoding Na+/H+antiporters, nhaS1-5 and sll0556. In this study, the function of NhaS3 was examined. NhaS3 was essential for gro...
Kiichi Kato, Ivan Gàlis, Ivan Gàlis, Shiori Suzuki, Satoshi Araki, Taku Demura, Marie Claire Criqui, Thomas Potuschak, Pascal Genschik, Hiroo Fukuda, Hiroo Fukuda, Ken Matsuoka, Ken Matsuoka, Masaki Ito
Plant Physiology   149(4) 1945-1957   Apr 2009
Many G2/M phase-specific genes in plants contain mitosis-specific activator (MSA) elements, which act as G2/M phasespecific enhancers and bind with R1R2R3-Myb transcription factors. Here, we examined the genome-wide effects of NtmybA2 overexpressi...
Tsubasa Shoji, Koji Inai, Yoshiaki Yazaki, Yasutaka Sato, Hisabumi Takase, Hisabumi Takase, Nobukazu Shitan, Kazufumi Yazaki, Yumi Goto, Kiminori Toyooka, Ken Matsuoka, Ken Matsuoka, Takashi Hashimoto
Plant Physiology   149(2) 708-718   Feb 2009
Nicotine is a major alkaloid accumulating in the vacuole of tobacco (Nicotiana tabacum), but the transporters involved in the vacuolar sequestration are not known. We here report that tobacco genes (NtMATE1 and NtMATE2) encoding transporters of th...
Mst Nasrin Akhter Banu, Md Anamul Hoque, Megumi Watanabe-Sugimoto, Ken Matsuoka, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata
Journal of Plant Physiology   166 146-156   Jan 2009
Salt stress causes oxidative damage and cell death in plants. Plants accumulate proline and glycinebetaine (betaine) to mitigate detrimental effects of salt stress. The aim of this study was to investigate the protective effects of proline and bet...
Kiminori Toyooka, Yumi Goto, Satoru Asatsuma, Satoru Asatsuma, Masato Koizumi, Toshiaki Mitsui, Ken Matsuoka, Ken Matsuoka
Plant Cell   21(4) 1212-1229   Jan 2009
Secretory proteins and extracellular glycans are transported to the extracellular space during cell growth. These materials are carried in secretory vesicles generated at the trans-Golgi network (TGN). Analysis of the mammalian post-Golgi secretor...
Kiminori Toyooka, Ken Matsuoka, Ken Matsuoka
Plant Signaling and Behavior   4    Jan 2009
Exo- and endocytotic membrane trafficking is an essential process for transport of secretory proteins, extracellular glycans, transporters and lipids in plant cells. Using secretory carrier membrane protein 2 (SCAMP2) as a marker for secretory ves...
Ken Matsuoka
Methods in enzymology   451 541-555   Dec 2008
Autophagy is induced under nutrient-deficient conditions in both growing tobacco BY-2 cultured cells as well as Arabidopsis and others intact plants. The fluorescent protein-tagged structural protein for autophagosomes, the Atg8 protein, allows no...
Daniel J. Klionsky, Daniel J. Klionsky, Hagai Abeliovich, Patrizia Agostinis, Devendra K. Agrawal, Gjumrakch Aliev, David S. Askew, Misuzu Baba, Eric H. Baehrecke, Ben A. Bahr, Andrea Ballabio, Bruce A. Bamber, Diane C. Bassham, Ettore Bergamini, Xiaoning Bi, Martine Biard-Piechaczyk, Janice S. Blum, Dale E. Bredesen, Jeffrey L. Brodsky, John H. Brumell, Ulf T. Brunk, Wilfried Bursch, Nadine Camougrand, Eduardo Cebollero, Francesco Cecconi, Yingyu Chen, Lih Shen Chin, Augustine Choi, Charleen T. Chu, Jongkyeong Chung, Peter G.H. Clarke, Robert S.B. Clark, Steven G. Clarke, Corinne Clavé, John L. Cleveland, Patrice Codogno, María I. Colombo, Ana Cotomontes, James M. Cregg, Ana Maria Cuervo, Jayanta Debnath, Francesca Demarchi, Patrick B. Dennis, Phillip A. Dennis, Vojo Deretic, Rodney J. Devenish, Federica Di Sano, J. Fred Dice, Marian DiFiglia, Savithramma Dinesh-Kumar, Clark W. Distelhorst, Mojgan Djavaheri-Mergny, Frank C. Dorsey, Wulf Dröge, Michel Dron, William A. Dunn, Michael Duszenko, N. Tony Eissa, Zvulun Elazar, Audrey Esclatine, Eeva Liisa Eskelinen, László Fésüs, Kim D. Finley, José M. Fuentes, Juan Fueyo, Kozo Fujisaki, Brigitte Galliot, Fen Biao Gao, David A. Gewirtz, Spencer B. Gibson, Antje Gohla, Alfred L. Goldberg, Ramon Gonzalez, Cristina González-Estévez, Sharon Gorski, Roberta A. Gottlieb, Dieter Häussinger, You Wen He, Kim Heidenreich, Joseph A. Hill, Maria Høyer-Hansen, Xun Hu, Wei Pang Huang, Akiko Iwasaki, Marja Jäättelä, William T. Jackson, Xuejun Jiang, Shengkan Jin, Terje Johansen, Jae U. Jung, Motoni Kadowaki, Chanhee Kang, Ameeta Kelekar, David H. Kessel, Jan A.K.W. Kiel, Pyo Kim Hong, Adi Kimchi, Timothy J. Kinsella, Kirill Kiselyov, Katsuhiko Kitamoto
Autophagy   4 151-175   Feb 2008
Research in autophagy continues to accelerate,1and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews ha...
Shin Hamamoto, Junichiro Marui, Ken Matsuoka, Kyohei Higashi, Kazuei Igarashi, Tsuyoshi Nakagawa, Teruo Kuroda, Yasuo Mori, Yoshiyuki Murata, Yoichi Nakanishi, Masayoshi Maeshima, Isamu Yabe, Nobuyuki Uozumi
Journal of Biological Chemistry   283 1911-1920   Jan 2008
The tonoplast K+ membrane transport system plays a crucial role in maintaining K+ homeostasis in plant cells. Here, we isolated cDNAs encoding a two-pore K+ channel (NtTPK1) from Nicotiana tabacum cv. SR1 and cultured BY-2 tobacco cells. Two of th...
Hiroki Ukitsu, Hiroki Ukitsu, Takashi Kuromori, Kiminori Toyooka, Yumi Goto, Ken Matsuoka, Ken Matsuoka, Eiji Sakuradani, Sakayu Shimizu, Asako Kamiya, Yuko Imura, Masahiro Yuguchi, Takuji Wada, Takashi Hirayama, Takashi Hirayama, Kazuo Shinozaki
Plant and Cell Physiology   48 1524-1533   Nov 2007
In transposon-tagged lines of Arabidopsis we found two new mutants, cof1-1 and cof1-2 (cuticular defect and organ fusion), that show the phenotype of wilting when grown in soil, organ fusion of rosette leaves and infertility. Toluidine blue testin...
Tomonori Shinya, Tomonori Shinya, Tomonori Shinya, Ivan Gális, Ivan Gális, Tomoko Narisawa, Mami Sasaki, Hiroo Fukuda, Hideaki Matsuoka, Mikako Saito, Ken Matsuoka, Ken Matsuoka
Plant and Cell Physiology   48 1404-1413   Oct 2007
We previously demonstrated that a β-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression ...
Tsuyoshi Nakagawa, Takayuki Kurose, Takeshi Hino, Katsunori Tanaka, Makoto Kawamukai, Yasuo Niwa, Kiminori Toyooka, Ken Matsuoka, Ken Matsuoka, Tetsuro Jinbo, Tetsuya Kimura
Journal of Bioscience and Bioengineering   104(1) 34-41   Jul 2007
We developed a new series of binary vectors useful for Gateway cloning to facilitate transgenic experiments in plant biotechnology. The new system, Gateway Binary Vectors (pGWBs) realized efficient cloning, constitutive expression using the caulif...
Tomonori Shinya, Kazunari Hanai, Ivan Gális, Kaoru Suzuki, Ken Matsuoka, Hideaki Matsuoka, Mikako Saito
Biochemical and Biophysical Research Communications   353 311-317   Feb 2007
Tobacco BY-2 class IV chitinases (TBC-1, TBC-3) were rapidly and transiently induced by the β-1,3-, 1,6-glucan elicitor from Alternaria alternata 102 (AaGlucan). The full-length cDNA and 5′-flanking region of a gene encoding class IV chitinases we...
Yohei Matsuki, Mari Ohmura-Hoshino, Eiji Goto, Masami Aoki, Mari Mito-Yoshida, Mika Uematsu, Takanori Hasegawa, Haruhiko Koseki, Osamu Ohara, Osamu Ohara, Manabu Nakayama, Kiminori Toyooka, Ken Matsuoka, Ken Matsuoka, Hak Hotta, Akitsugu Yamamoto, Satoshi Ishido, Satoshi Ishido
EMBO Journal   26 846-854   Feb 2007
The presence of post-translational regulation of MHC class II (MHC II) under physiological conditions has been demonstrated recently in dendritic cells (DCs) that potently function as antigen-presenting cells (APCs). Here, we report that MARCH-I, ...
I. Galis, K. Matsuoka
Salicylic Acid: A Plant Hormone   371-396   Jan 2007
© 2007 Springer. All rights reserved. Tobacco has played historically important role in the discovery and functional analysis of salicylic acid (SA) as a plant hormone. Using this model, it was demonstrated for the first time that tobacco mosaic v...
Naoki Kasukabe, Megumi Watanabe-Sugimoto, Ken Matsuoka, Ken Matsuoka, Eiji Okuma, Ichiro Obi, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata, Tadaaki Kakutani
Plant and Cell Physiology   47 1674-1677   Dec 2006
The expression of plasma membrane K+ channels of NaCl-adapted tobacco suspension cells and effects of extracellular Ca2+ on plasma membrane K+ channels were investigated. Reverse transcription-PCR (RT-PCR) analysis showed that expression of TORK1,...
Tomonori Shinya, Tomonori Shinya, Rozenn Ménard, Ikuko Kozone, Hideaki Matsuoka, Naoto Shibuya, Serge Kauffmann, Ken Matsuoka, Mikako Saito, Mikako Saito
FEBS Journal   273 2421-2431   Jun 2006
A novel elicitor that induces chitinases in tobacco BY-2 cells was isolated from Alternaria alternata 102. Six other fungi, including A. alternata IFO 6587, could not induce, or weakly induce chitinase activity. The purified elicitor was soluble i...
Ivan Gális, Petr Šimek, Tomoko Narisawa, Mami Sasaki, Tatsuya Horiguchi, Hiroo Fukuda, Hiroo Fukuda, Ken Matsuoka
Plant Journal   43 573-592   May 2006
Target metabolic and large-scale transcriptomic analyses of tobacco (Nicotiana tabacum L.) Bright Yellow-2 (BY-2) cells were employed to identify novel gene(s) involved in methyl jasmonate (MJ)-dependent function in plants. At the metabolic level,...
Yi He, Reiko Sugiura, Reiko Sugiura, Yan Ma, Ayako Kita, Ayako Kita, Lu Deng, Kaoru Takegawa, Ken Matsuoka, Hisato Shuntoh, Takayoshi Kuno
Genes to Cells   11 207-221   Mar 2006
We have previously isolated ypt3-i5 mutant and showed that Ypt3 GTPase functions in the fission yeast secretory pathway. Here, the same genetic screen led to the isolation of ryh1-i6, a mutant allele of the ryh1+ gene encoding a homolog of Rab6. T...
K. Matsuoka, I. Galis
Biotechnology in Agriculture and Forestry   58 293-311   Jan 2006
Kiminori Toyooka, Yuji Moriyasu, Yumi Goto, Masaki Takeuchi, Hiroo Fukuda, Ken Matsuoka, Ken Matsuoka
Autophagy   2 96-106   Jan 2006   [Refereed]
When a fusion protein of cytochrome b5 (Cyt b5) and the red fluorescent protein (RFP) are expressed in tobacco BY-2 cells, the expressed protein forms intracellular aggregates that emit red fluorescence. When such cells are grown to the stationary...
H. Matsuoka, H. Matsuoka, Y. Yamada, Y. Yamada, K. Matsuoka, M. Saito, M. Saito
Biotechnology in Agriculture and Forestry   58 339-346   Jan 2006
© Springer-Verlag Berlin Heidelberg 2006. Single-cell analysis of BY-2 that can solely be performed with microinjection hasbeendemonstrated. This shows thatmicroinjection is feasible asapractical methodeven for small cellsnogreater than 50µm in di...
Daisuke Yamauchi, Daisuke Yamauchi, Keita Sutoh, Hiromi Kanegae, Tatsuya Horiguchi, Ken Matsuoka, Hiroo Fukuda, Masamitsu Wada, Masamitsu Wada
Journal of Plant Research   118 223-227   Jul 2005
The analysis of expressed sequences from a diverse set of plant species has fueled the increase in understanding of the complex molecular mechanisms underlying plant growth regulation. While representative data sets can be found for the major bran...
Masami Shimizu, Tomohiro Igasaki, Tomohiro Igasaki, Makiko Yamada, Koji Yuasa, Jyunko Hasegawa, Tetsuji Kato, Hironaka Tsukagoshi, Kenzo Nakamura, Hiroo Fukuda, Ken Matsuoka, Ken Matsuoka
Plant Journal   42 877-889   Jun 2005
Many secretory and several vacuolar proteins in higher plants contain hydroxylated proline residues. In many cases, hydroxyprolines in proteins are glycosylated with either arabinogalactan or oligoarabinose. We have previously shown that a sporami...
Takamasa Suzuki, Takamasa Suzuki, Sakiko Nakajima, Soichi Inagaki, Makiko Hirano-Nakakita, Ken Matsuoka, Taku Demura, Hiroo Fukuda, Atsushi Morikami, Kenzo Nakamura
Plant and Cell Physiology   46 736-742   May 2005
TONSOKU(TSK)/MGOUN3/BRUSHY1 of Arabidopsis thaliana encodes a nuclear leucine-glycine-aspargine (LGN) domain protein implicated to be involved in genome maintenance, and mutants with defects in TSK show a fasciated stem with disorganized meristem ...
Koji Yuasa, Kiminori Toyooka, Hiroo Fukuda, Ken Matsuoka
Plant Journal   41: 81-94 81-94   Jan 2005
We cloned a novel prolyl 4-hydroxylase (PH; EC homolog cDNA from tobacco (Nicotiana tabacum) BY-2 cells based on expression sequence tag information. Like other PHs, this tobacco PH polypeptide has two conserved histidine residues, and ...
Chihiro Takatsuka, Yuko Inoue, Ken Matsuoka, Yuji Moriyasu
Plant and Cell Physiology   45 265-274   Mar 2004
Tobacco (Nicotiana tabacum) culture cells perform autophagy and degrade cellular proteins in response to sucrose starvation. When protein degradation is blocked by the cysteine protease inhibitor E-64c, lysosomes containing particles of cytoplasm ...
Ken Matsuoka, Taku Demura, Ivan Galis, Tatsuya Horiguchi, Mami Sasaki, Gen Tashiro, Hiroo Fukuda, Hiroo Fukuda
Plant and Cell Physiology   45: 1280-1289 1280-1289   Jan 2004
To understand how plant cell changes gene expression during cell division and after termination of cell division, we analyzed the change of gene expression during the growth of tobacco BY-2 cell lines using a cDNA microarray, which contained about...
Hiroo Fukuda, Taku Demura, Ken Matsuoka
Nippon Nogeikagaku Kaishi   76 42-44   Jan 2002
Ken Matsuoka
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   47 1760-1764   Jan 2002
Ken Matsuoka, Ken Matsuoka, Randy Schekman, Lelio Orci, John E. Heuser
Proceedings of the National Academy of Sciences of the United States of America   98 13705-13709   Nov 2001
The spatial arrangement of COPII coat protein subunits was analyzed by crosslinking to an artificial membrane surface and by electron microscopy of coat proteins and coated vesicle surfaces. The efficiency of COPII subunit crosslinking to phosphol...
Ken Matsuoka, Randy Schekman
Methods   20(20) 417-428   2001
We have established systems that reconstitute the biogenesis of coated transport vesicles with liposomes made of pure lipids and purified coat proteins. Optimization of the lipid composition in the liposomes allowed the efficient binding of both c...
Sharif U. Ahmed, Sharif U. Ahmed, Enrique Rojo, Valentina Kovaleva, Sridhar Venkataraman, Sridhar Venkataraman, James E. Dombrowski, James E. Dombrowski, Ken Matsuoka, Natasha V. Raikhel, Natasha V. Raikhel, Natasha V. Raikhel
Journal of Cell Biology   149 1335-1344   Jun 2000
Many soluble plant vacuolar proteins are sorted away from secreted proteins into small vesicles at the trans-Golgi network by transmembrane cargo receptors. Cleavable vacuolar sorting signals include the NH2-terminal propeptide (NTPP) present in s...
Ken Matsuoka, Kenzo Nakamura
Plant Molecular Biology   41 825-835   Dec 1999
The N-terminal propeptide of the sporamin precursor contains vacuolar targeting information within the Asn-26/Pro-27/Ile-28/Arg-29/Leu-30 (NPIRL) sequence. An Agrobacterium-mediated transient expression assay with tobacco BY-2 cells was employed t...
K. Matsuoka
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   44 676-682   Jan 1999
Ken Matsuoka, Jean Marc Neuhaus
Journal of Experimental Botany   50 165-174   Jan 1999
Vacuolar proteins are synthesized and translocated into the endoplasmic reticulum and transported to the vacuoles through the secretory pathway. Three different types of vacuolar sorting signals have been identified, carried by N- or C-terminal pr...
Yasuhiro Koide, Ken Matsuoka, Masa Aki Ohto, Kenzo Nakamura, Kenzo Nakamura
Plant and Cell Physiology   40 1152-1159   Jan 1999
Two types of vacuolar sorting signals (VSSs), an asparagine-proline-isoleucine-arginine-leucine (NPIRL)-related VSS in the N-terminal propeptides (NTPPs) and a C-terminal VSS in the C-terminal propeptides (CTPPs), function differently in plant cel...
A. Spang, K. Matsuoka, S. Hamamoto, R. Schekman, L. Orci
Proceedings of the National Academy of Sciences of the United States of America   95 11199-11204   Sep 1998
Synthetic coat protein complex I (COPI)-coated vesicles form spontaneously from large (≃300 nm in diameter), chemically defined liposomes incubated with coatomer, Arf1p, and guanosine 5'-[γ-thio]triphosphate. Coated vesicles are 40-70 nm in diamet...
Ken Matsuoka, Lelio Orci, Mylène Amherdt, Sebastian Y. Bednarek, Sebastian Y. Bednarek, Susan Hamamoto, Randy Schekman, Thomas Yeung, Thomas Yeung
Cell   93: 263-275 263-275   Apr 1998
COPII vesicle formation requires only three coat assembly subunits: Sarl p, Sac13/31 p, and Sec23/24p. PI 4-phosphate or PI 4,5-bisphosphate is required for the binding of these proteins to liposomes. The GTP-bound form of Sar1p recruits Sec23/24p...
Ken Matsuoka, Yasujiro Morimitsu, Koji Uchida, Randy Schekman
Molecular Cell   2: 703-708 703-708   Jan 1998
COPII proteins are required to create transport vesicles and to select cargo molecules for transit from the ER. A reconstituted liposome budding reaction was used to detect the capture and concentration of membrane-associated v-SNARE molecules int...
Ken Matsuoka, Sebastian Y. Bednarek
Current Opinion in Plant Biology   1 463-469   Jan 1998
The secretory pathway plays a central role in plant development and morphogenesis. Storage protein deposition, plant cell division and the expansion of the plasma membrane and extracellular matrix all require the synthesis and trafficking of membr...
R. Schekman, Susan Hamamoto, M. Kuehn, K. Matsuoka, T. Yeung, L. Orci
FASEB Journal   11    Dec 1997
The cytosolic yeast proteins Secl3-31p, Sec23/24p, and the small GTP binding protein Sarlp form the membrane coat termed COPII. Purified COPII components and GTP form anterograde transport vesicles directly from the endoplasmic reticulum. Cargo ca...
Ken Matsuoka, Ken Matsuoka, Tatsuji Higuchi, Masayoshi Maeshima, Kenzo Nakamura
Plant Cell   9 533-546   Apr 1997
In plant cells, vacuolar matrix proteins are separated from the secretory proteins at the Golgi complex for transport to the vacuoles. To investigate the involvement of vacuolar-type ATPase (V-ATPase) in the vacuolar targeting of soluble proteins,...
Yasuhiro Koide, Yasuhiro Koide, Hideyuki Hirano, Hideyuki Hirano, Ken Matsuoka, Kenzo Nakamura, Kenzo Nakamura
Plant Physiology   114 863-870   Jan 1997
An asparagine-proline-isoleucine-arginine-leucine (NPIRL) and its related sequences in the N-terminal propeptides (NTPP) of several plant vacuolar proteins, including that of sporamin from sweet potato (SPO) function as vacuole-targeting determina...
Shunsuke Imanishi, Kyoko Kito-Nakamura, Ken Matsuoka, Atsushi Morikami, Kenzo Nakamura
Plant and Cell Physiology   38 643-652   Jan 1997
Treatment of sweet potato plants cultured in vitro with a vapor of methyl jasmonate (MeJA) induced an accumulation in leaves of a large amount of protein with an apparent molecular mass of 18 kDa. This protein, designated ipomoelin, was purified, ...
Yasushi Yoshioka, Yoshito Takahashi, Yoshito Takahashi, Ken Matsuoka, Kenzo Nakamura, Jun Koizumi, Mineo Kojima, Yasunori Machida, Yasunori Machida
Plant and Cell Physiology   37 782-789   Jan 1996
A simple system is described for detection of the transfer of T-DNA from Agrobacterium cells to suspension-cultured tobacco BY-2 cells. A modified reporter gene for β-glucuronidase (GUS) that contained an intron sequence was introduced into the T-...
Ken Matsuoka, Ken Matsuoka, Diane C. Bassham, Natasha V. Raikhel, Kenzo Nakamura
Journal of Cell Biology   130(6) 1307-1318   Sep 1995
Vacuolar matrix proteins in plant cells are sorted from the secretory pathway to the vacuoles at the Golgi apparatus. Previously, we reported that the NH2-terminal propeptide (NTPP) of the sporamin precursor and the COOH- terminal propeptide (CTPP...
Shin Takeda, Yasuo Kowyama, Yuka Takeuchi, Ken Matsuoka, Mikio Nishimura
Plant and Cell Physiology   36 321-333   Jan 1995
Two major proteins of tuberous roots of sweet potato, sporamin and rβ-amylase, were detected in storage parenchyma cells, which contain a large amount of starch. In both the leaves and petioles of sweet potato, the sucrose-induced accumulation of ...
Ken Matsuoka, Natsu Watanabe, Kenzo Nakamura
The Plant Journal   8 877-889   Jan 1995
Sporamin, a vacuolar protein of the sweet potato, is synthesized as a precursor that contains signal peptide and an N‐terminal propeptide that functions as a vacuolar targeting determinant. Sporamin, when expressed in tobacco cells, migrated as sm...
Atsushi Ishikawa, Shozo Ohta, Ken Matsuoka, Tsukaho Hattori, Kenzo Nakamura
Plant and Cell Physiology   35 303-312   Dec 1994
Potato tubers contain a complex group of proteins of 20 to 24 kDa that exhibit homology to Kunitz-type proteinase inhibitors. We isolated three cDNAs and two genomic clones that encode members of the potato Kunitz-type proteinase inhibitor (PKPI) ...
Tetsuya Kimura, Noriyuki Kitamoto, Ken Matsuoka, Kenzo Nakamura, Yuzuru Iimura, Yukio Kito
Gene   137 265-270   Dec 1993
The HMK gene, encoding a killer toxin (HMK) of Hansenula mrakii strain IFO 0895, and the HSK gene, encoding a killer toxin (HSK) of H. saturnus strain IFO 0117, were cloned and sequenced. The HMK and HSK genes encode precursors to killer toxins of...
Kenzo Nakamura, Ken Matsuoka
Plant Physiology   101 1-5   Jan 1993
Martin R. Schroeder, Olga N. Borkhsenious, Ken Matsuoka, Kenzo Nakamura, Natasha V. Raikhel
Plant Physiology   101 451-458   Jan 1993
Various targeting motifs have been identified for plant proteins delivered to the vacuole. For barley (Hordeum vulgare) lectin, a typical Gramineae lectin and defense-related protein, the vacuolar information is contained in a carboxyl-terminal pr...
Ken Matsuoka, Kenzo Nakamura
Plant and Cell Physiology   33 453-462   Jun 1992
The propeptide of a precursor to sporamin, a storage protein of sweet potato, is required for targeting of sporamin to the vacuole in transformed tobacco cells (Matsuoka and Nakamura 1991). A fusion gene consisting of an inducible GAL 10 promoter ...
K. Matsuoka, K. Nakamura
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   37 1172-1177   May 1992
Hitoshi Onouchi, Kumi Yokoi, Chiyoko Machida, Hiroaki Matsuzaki, Hiroaki Matsuzaki, Yasuji Oshima, Yasuji Oshima, Ken Matsuoka, Ken Matsuoka, Kenzo Nakamura, Kenzo Nakamura, Yasunori Machida
Nucleic Acids Research   19 6373-6378   Dec 1991
Recombinase encoded by the R gene of pSR1 of Zygosaccharomyces rouxii mediates reciprocal recombination between two specific recombination sites (RSs) to induce excision or inversion of the DNA segment that is flanked by the RSs. We report here th...
Ken Matsuoka, Kenzo Nakamura
Proceedings of the National Academy of Sciences of the United States of America   88 834-838   Jan 1991
Sporamin is a protein without glycans that accumulates in large quantities in the vacuoles of the tuberous root of the sweet potato. It is synthesized as a prepro precursor with an N-terminal extension composed of a 21-amino-acid signal peptide an...
Ken Matsuoka, Shogo Matsumoto, Tsukaho Hattori, Yasunori Machida, Kenzo Nakamura
Journal of Biological Chemistry   265 19750-19757   Nov 1990
Sporamin, the tuberous root storage protein of the sweet potato, which is localized in vacuoles, is synthesized as a prepro-precursor with an N-terminal sequence of amino acids that includes a signal peptide and an additional pro-segment of 16 ami...
Tsukaho Hattori, Ken Matsuoka, Kenzo Nakamura
Agricultural and Biological Chemistry   52 1057-1059   Jan 1988


Toshiyuki Nagata, Ken Matsuoka, Dirk Inzé
Biotechnology in Agriculture and Forestry   58    Jan 2006

Books etc

Tobacco BY-2 Cells: From Cellular Dynamics to Omics
2006   ISBN:978-3-540-32674-8