2021年12月
Synthetic mycobacterial diacyl trehaloses reveal differential recognition by human T cell receptors and the C-type lectin Mincle
Scientific Reports
- 巻
- 11
- 号
- 1
- 開始ページ
- 2010
- 終了ページ
- 2010
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41598-021-81474-3
The cell wall of Mycobacterium tuberculosis is composed of diverse glycolipids which potentially interact with the human immune system. To overcome difficulties in obtaining pure compounds from bacterial extracts, we recently synthesized three forms of mycobacterial diacyltrehalose (DAT) that differ in their fatty acid composition, DAT , DAT , and DAT . To study the potential recognition of DATs by human T cells, we treated the lipid-binding antigen presenting molecule CD1b with synthetic DATs and looked for T cells that bound the complex. DAT - and DAT -treated CD1b tetramers were recognized by T cells, but DAT -treated CD1b tetramers were not. A T cell line derived using CD1b-DAT tetramers showed that there is no cross-reactivity between DATs in an IFN-γ release assay, suggesting that the chemical structure of the fatty acid at the 3-position determines recognition by T cells. In contrast with the lack of recognition of DAT by human T cells, DAT but not DAT or DAT , activates Mincle. Thus, we show that the mycobacterial lipid DAT can be both an antigen for T cells and an agonist for the innate Mincle receptor, and that small chemical differences determine recognition by different parts of the immune system. 1 2 3 1 2 3 2 3 3, 1 2
- リンク情報
-
- DOI
- https://doi.org/10.1038/s41598-021-81474-3
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/33479373
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820438
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85099937144&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85099937144&origin=inward
- ID情報
-
- DOI : 10.1038/s41598-021-81474-3
- eISSN : 2045-2322
- PubMed ID : 33479373
- PubMed Central 記事ID : PMC7820438
- SCOPUS ID : 85099937144