MISC

2010年6月

Correlation of Cell Adhesive Behaviors on Superhydrophobic, Superhydrophilic, and Micropatterned Superhydrophobic/Superhydrophilic Surfaces to Their Surface Chemistry

LANGMUIR
  • Takahiro Ishizaki
  • ,
  • Nagahiro Saito
  • ,
  • Osamu Takai

26
11
開始ページ
8147
終了ページ
8154
記述言語
英語
掲載種別
DOI
10.1021/la904447c
出版者・発行元
AMER CHEMICAL SOC

A micropatterned superhydrophobic/superhydrophilic surface was successfully fabricated by plasma CVD and VUV irradiation. Physicochemical properties of the superhydrophobic, superhydrophilic, and superhydrophobic/superhydrophilic surfaces were investigated. The roughness structures on the superhydrophilic surface remained intact compared to those of the superhydrophobic surface. The micropatterned superhydrophobic/superhydrophilic surface was used as a scaffold of cell culture. On the micropatterned surface, the cells attached to the superhydrophilic regions in a highly selective manner. forming circular microarrays of the cells corresponding to the pattern. On the micropatterned surface with pattern distances of 200 mu m between superhydrophilic regions, the cells adhered on the superhydrophilic regions and partly extended to the neighboring cells. In contrast, when the pattern distances between the superhydrophilic regions were more than 400 mu m, the cells did not extend to the neighboring cells. Cell adhesion behaviors on superhydrophobic and superhydrophilic surfaces were also examined. The cells adhered and proliferated on both superhydrophobic and superhydrophilic surfaces. However, on the superhydrophobic surface, constant contact to facilitate cell division and proliferation was required. On the other hand, the cells easily adhered and proliferated on the superhydrophilic surface immediately alter seeding. These differences in cell adhesion behavior induced site-selective cell adhesion on the superhydrophilic regions. Furthermore, protein adsorption behavior that plays an important role in cell adhesion on flat hydrophobic and hydrophilic surface was also examined. The amounts of the protein adsorption on the flat hydrophilic surface were much greater than those on the flat hydrophobic surface.

リンク情報
DOI
https://doi.org/10.1021/la904447c
CiNii Articles
http://ci.nii.ac.jp/naid/80021078746
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20131757
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000277928100066&DestApp=WOS_CPL
ID情報
  • DOI : 10.1021/la904447c
  • ISSN : 0743-7463
  • CiNii Articles ID : 80021078746
  • PubMed ID : 20131757
  • Web of Science ID : WOS:000277928100066

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