論文

査読有り
2006年5月

Examination of drug resistance activity of human TAP-like (ABCB9) expressed in yeast

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • A Ohashi-Kobayashi
  • ,
  • K Ohashi
  • ,
  • WB Du
  • ,
  • H Omote
  • ,
  • R Nakamoto
  • ,
  • M Al-shawi
  • ,
  • M Maeda

343
2
開始ページ
597
終了ページ
601
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2006.03.002
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

A half-type ABC transporter, human TAP-like (hTAPL) tagged with histidine cluster, was expressed in budding yeast protease-deficient strain BJ5457, and the effect of expression for resistance to peptide compounds including antibiotics and proteinase inhibitor was examined. Among these Compounds, the yeast expressing hTAPL exhibits high sensitivity to valinomycin, a monovalent cation ionophore. A mutation in Walker A motif, which lost ATP-binding activity of hTAPL, eliminated the enhanced sensitivity to valinomycin. These findings suggest that the transport activity of hTAPL is important for conferring high valinomycin-sensitive phenotype to yeast. (c) 2006 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2006.03.002
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/16554024
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000236659800035&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.bbrc.2006.03.002
  • ISSN : 0006-291X
  • PubMed ID : 16554024
  • Web of Science ID : WOS:000236659800035

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