SUGIURA Shinji

J-GLOBAL         Last updated: Nov 13, 2018 at 13:17
 
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Name
SUGIURA Shinji
Affiliation
National Institute of Advanced Industrial Science and Technology
ORCID ID
0000-0002-9655-2189

Published Papers

 
Shibuta M, Tamura M, Kanie K, Yanagisawa M, Matsui H, Satoh T, Takagi T, Kanamori T, Sugiura S, Kato R
Journal of bioscience and bioengineering   126(5) 653-660   Nov 2018   [Refereed]
Tashiro S, Le MNT, Kusama Y, Nakatani E, Suga M, Furue MK, Satoh T, Sugiura S, Kanamori T, Ohnuma K
Journal of bioscience and bioengineering   126(3) 379-388   Sep 2018   [Refereed]
Kanamori T, Sugiura S, Sakai Y
Drug metabolism and pharmacokinetics   33(1) 40-42   Feb 2018   [Refereed]
Kondo Y, Hattori K, Tashiro S, Nakatani E, Yoshimitsu R, Satoh T, Sugiura S, Kanamori T, Ohnuma K
Journal of bioscience and bioengineering   124(2) 234-241   Aug 2017   [Refereed]
T. Satoh, S. Sugiura, K. Shin, R. Onuki-Nagasaki, S. Ishida, K. Kikuchi, M. Kakiki, T. Kanamori
Lab on a Chip   18 115-125   Jan 2017
© 2018 The Royal Society of Chemistry. This paper reports a multi-throughput multi-organ-on-a-chip system formed on a pneumatic pressure-driven medium circulation platform with a microplate-sized format as a novel type of microphysiological system...
Tamura M, Sugiura S, Takagi T, Satoh T, Sumaru K, Kanamori T, Okada T, Matsui H
PloS one   12(6) e0179372   2017   [Refereed]
Sugiura S, Cha JM, Yanagawa F, Zorlutuna P, Bae H, Khademhosseini A
Journal of tissue engineering and regenerative medicine   10(8) 690-699   Aug 2016   [Refereed]
Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Kanamori
Regenerative Therapy   3 45-57   Mar 2016
© 2016 The Japanese Society for Regenerative Medicine. The development of biologically relevant three-dimensional (3D) tissue constructs is essential for the alternative methods of organ transplantation in regenerative medicine, as well as the dev...
T. Satoh, G. Narazaki, R. Sugita, H. Kobayashi, S. Sugiura, T. Kanamori
Lab on a Chip   16 2339-2348   Jan 2016
© 2016 The Royal Society of Chemistry. Here, we report a pneumatic pressure-driven microfluidic device capable of multi-throughput medium circulation culture. The circulation culture system has the following advantages for application in drug disc...
Tamura M, Yanagawa F, Sugiura S, Takagi T, Sumaru K, Kanamori T
Scientific reports   5 15060   Oct 2015   [Refereed]
Yanagawa F, Mizutani T, Sugiura S, Takagi T, Sumaru K, Kanamori T
Colloids and surfaces. B, Biointerfaces   126 575-579   Feb 2015   [Refereed]
Yanagawa F, Sugiura S, Takagi T, Sumaru K, Camci-Unal G, Patel A, Khademhosseini A, Kanamori T
Advanced healthcare materials   4(2) 246-254   Jan 2015   [Refereed]
Hattori K, Munehira Y, Kobayashi H, Satoh T, Sugiura S, Kanamori T
Journal of bioscience and bioengineering   118(3) 327-332   Sep 2014   [Refereed]
Yamada R, Hattori K, Tachikawa S, Tagaya M, Sasaki T, Sugiura S, Kanamori T, Ohnuma K
Journal of bioscience and bioengineering   118(3) 315-322   Sep 2014   [Refereed]
Saeki D, Sugiura S, Kanamori T, Sato S, Ichikawa S
Journal of bioscience and bioengineering   118(2) 199-204   Aug 2014   [Refereed]
Sakai Y, Hattori K, Yanagawa F, Sugiura S, Kanamori T, Nakazawa K
Biotechnology journal   9(7) 971-979   Jul 2014   [Refereed]
Yoshimitsu R, Hattori K, Sugiura S, Kondo Y, Yamada R, Tachikawa S, Satoh T, Kurisaki A, Ohnuma K, Asashima M, Kanamori T
Biotechnology and bioengineering   111(5) 937-947   May 2014   [Refereed]
Tamura M, Yanagawa F, Sugiura S, Takagi T, Sumaru K, Matsui H, Kanamori T
Scientific reports   4 4793   May 2014   [Refereed]
Satoh T, Sugiura S, Sumaru K, Ozaki S, Gomi S, Kurakazu T, Oshima Y, Kanamori T
Biomicrofluidics   8(2) 024112   Mar 2014   [Refereed]
Taku Satoh, Kohei Kodama, Koji Hattori, Koji Hattori, Sosaku Ichikawa, Shinji Sugiura, Toshiyuki Kanamori
Journal of Chemical Engineering of Japan   47 841-847   Jan 2014
© 2014 the Society of Chemical Engineers, Japan. This paper reports a microfluidic droplet formation device suitable for manipulating small amounts of samples using a pressure-driven liquid-delivery system. In this system, a small volume (less tha...
Ryotaro Yamada, Koji Hattori, Motohiro Tagaya, Toru Sasaki, Daisuke Miyamoto, Kohji Nakazawa, Shinji Sugiura, Toshiyuki Kanamori, Kiyoshi Ohnuma
Plasma Medicine   4 165-176   Jan 2014
© 2014 by Begell House, Inc. Plasma treatment is an easy method of cleaning and hydrophilizing glass and polymer surfaces to facilitate adsorption of cell-adhesive proteins. Thus a patterned plasma treatment is useful for fabricating cell adhesion...
Hattori K, Sugiura S, Kanamori T
Methods in molecular biology (Clifton, N.J.)   1104 251-263   2014   [Refereed]
Hattori K, Sugiura S, Kanamori T
Journal of laboratory automation   18(6) 437-445   Dec 2013   [Refereed]
Koji Hattori, Ryosuke Yoshimitsu, Shinji Sugiura, Atsushi Maruyama, Kiyoshi Ohnuma, Toshiyuki Kanamori
RSC Advances   3 17749-17754   Oct 2013
We present a simple micropatterning process to create a closed microchamber array with an extracellular matrix (ECM). The process includes homogeneous coating of the array with the ECM, micropatterned decomposition by plasma oxidation with physica...
Shinji Sugiura, Toshiyuki Kanamori
Biochemical Engineering Journal   57 69-74   Nov 2011
Mass transport phenomena in cell culture can be formulated by using classical reaction-diffusion equations; however, in practice, it is difficult to solve these equations analytically. Here, we used computer simulation to solve these equations, an...
Sugiura S, Sakai Y, Nakazawa K, Kanamori T
Biomicrofluidics   5(2) 22202   Jun 2011   [Refereed]
Hattori K, Sugiura S, Kanamori T
Lab on a chip   11(2) 212-214   Jan 2011   [Refereed]
Sugiura S, Hattori K, Kanamori T
Analytical chemistry   82(19) 8278-8282   Oct 2010   [Refereed]
Saeki D, Sugiura S, Kanamori T, Sato S, Ichikawa S
Lab on a chip   10(17) 2292-2295   Sep 2010   [Refereed]
Hattori K, Sugiura S, Kanamori T
Biotechnology journal   5(5) 463-469   May 2010   [Refereed]
Daisuke Saeki, Daisuke Saeki, Daisuke Saeki, Shinji Sugiura, Toshiyuki Kanamori, Seigo Sato, Sosaku Ichikawa
Lab on a Chip   10(3) 357-362   Jan 2010
We developed a novel microfluidic device to prepare monodisperse water-in-oil-in-water (W/O/W) emulsions with an ultra-thin (<1 m) oil phase layer. This microfluidic device was composed of two microchannel junctions, one of which had a step struct...
Kikuchi K, Sumaru K, Edahiro J, Ooshima Y, Sugiura S, Takagi T, Kanamori T
Biotechnology and bioengineering   103(3) 552-561   Jun 2009   [Refereed]
Koji Hattori, Shinji Sugiura, Toshiyuki Kanamori
Lab on a Chip   9 1763-1772   Jan 2009
This paper reports a serial dilution microfluidic network composed of microchannels with a high fluidic-resistance ratio for generating linear concentration profiles as well as logarithmic concentration profiles spanning 3 and 6 orders of magnitud...
Shinji Sugiura, András Szilágyi, András Szilágyi, Kimio Sumaru, Koji Hattori, Toshiyuki Takagi, Genovéva Filipcsei, Miklós Zrínyi, Toshiyuki Kanamori
Lab on a Chip   9 196–198-198   Jan 2009
Novel on-chip fluid control strategies, on-demand formation of arbitrary microchannels and parallel control of multiple microvalves were successfully demonstrated by means of computer-controlled micropatterned light irradiation of a photoresponsiv...
Sugiura S, Imano W, Takagi T, Sakai K, Kanamori T
Biosensors & bioelectronics   24(5) 1135-1140   Jan 2009   [Refereed]
Saeki D, Sugiura S, Kanamori T, Sato S, Mukataka S, Ichikawa S
Langmuir : the ACS journal of surfaces and colloids   24(23) 13809-13813   Dec 2008   [Refereed]
Shinji Sugiura, Jun Ichi Edahiro, Kyoko Kikuchi, Kimio Sumaru, Toshiyuki Kanamori
Biotechnology and Bioengineering   100(6) 1156-1165   Aug 2008
This article reports a pressure-driven perfusion culture chip developed for parallel drug cytotoxicity assay. The device is composed of an 8 × 5 array of cell culture microchambers with independent perfusion microchannels. It is equipped with a si...
Sugiura S, Edahiro J, Sumaru K, Kanamori T
Colloids and surfaces. B, Biointerfaces   63(2) 301-305   Jun 2008   [Refereed]
Sugiura S, Kuroiwa T, Kagota T, Nakajima M, Sato S, Mukataka S, Walde P, Ichikawa S
Langmuir : the ACS journal of surfaces and colloids   24(9) 4581-4588   May 2008   [Refereed]
Saeki D, Sugiura S, Baba T, Kanamori T, Sato S, Mukataka S, Ichikawa S
Journal of colloid and interface science   320(2) 611-614   Apr 2008   [Refereed]
Shinji Sugiura, Shinji Sugiura, Tatsuya Oda, Yasuyuki Aoyagi, Mitsuo Satake, Nobuhiro Ohkohchi, Mitsutoshi Nakajima, Mitsutoshi Nakajima
Lab on a Chip   8 1255-1257   Jan 2008
We have used a laminar flow stream formed by a microfabricated nozzle array to prepare cell-encapsulated alginate gel micro-tubes, in which cells formed a cylindrical multi-cellular aggregate after cultivation for two weeks. © The Royal Society of...
Shinji Sugiura, Kimio Sumaru, Katsuhide Ohi, Kazuaki Hiroki, Toshiyuki Takagi, Toshiyuki Kanamori
Sensors and Actuators, A: Physical   140 176-184   Nov 2007
This paper presents independent control of multiple microvalves, which are opened by local light irradiation. The application of photoresponsive polymer gel, which was developed by our research group, to photoresponsive microvalve was systematical...
Kimio Sumaru, Shinji Sugiura, Toshiyuki Kanamori
Biochemical Engineering Journal   36 304-309   Oct 2007
In this study, we discuss diffusion based oxygen and glucose supplies in cell culture chips of three different setups, where cells are regularly arranged and cultivated, using oxygen and glucose transport models on the basis of the previous work. ...
Sugiura S, Abe T, Yamaura Y, Makino S
Die Naturwissenschaften   94(8) 703-707   Aug 2007   [Refereed]
András Szilágyi, András Szilágyi, Kimio Sumaru, Shinji Sugiura, Toshiyuki Takagi, Toshio Shinbo, Miklós Zrínyi, Toshiyuki Kanamori
Chemistry of Materials   19 2730-2732   May 2007
The quick photoresponse of thin hydrogel layers composed of thermoresponsive poly(N-isopropylacrylamide) (pNIPAAm) with an acrylated SP chromophore incorporated in the polymer backbone were analyzed. Drastic photoinduced volume change of the hydro...
Sugiura S, Oda T, Aoyagi Y, Matsuo R, Enomoto T, Matsumoto K, Nakamura T, Satake M, Ochiai A, Ohkohchi N, Nakajima M
Biomedical microdevices   9(1) 91-99   Feb 2007   [Refereed]
Enomoto T, Oda T, Aoyagi Y, Sugiura S, Nakajima M, Satake M, Noguchi M, Ohkohchi N
Cancer research   66(23) 11131-11139   Dec 2006   [Refereed]
Shinji Sugiura, Shinji Sugiura, Shinji Sugiura, Tatsuya Oda, Tatsuya Oda, Yasuhiko Izumida, Yasuyuki Aoyagi, Mitsuo Satake, Atsushi Ochiai, Nobuhiro Ohkohchi, Mitsutoshi Nakajima
Biomaterials   26(16) 3327-3331   Jun 2005
Size-controlled small (i.e. less than 300 μm) polyelectrolyte complex gel beads are urgently desired for wide-spread application, including use in medical, pharmaceutical, and bioengineering fields. However, it was impossible to obtain smaller bea...
Shinji Sugiura, Mitsutoshi Nakajima, Minoru Seki
Industrial and Engineering Chemistry Research   43 8233-8238   Dec 2004
Microchannel (MC) emulsification is a novel technique for producing monodisperse emulsions. We previously proposed a droplet diameter prediction model for simple MC structures consisting of a narrow channel and a slitlike terrace. In this study, t...

Misc

 
Tatsuto Ono, Shinji Sugiura, Teruo Fujii, Yasuyuki Sakai, Hiroshi Kimura
MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science   2018-January 1-3   Feb 2018
© 2017 IEEE. In this study, we developed a microfluidic device with metabolism function during pharmacokinetic study. We reproduced physiological parameters such as an organ volume ratio and a blood flow ratio on the device. The ratios are replica...
Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori
2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015      Mar 2016
© 2015 IEEE. This paper reports a novel click-crosslinkable and photodegradable gelatin hydrogels, which is useful for micropatterned three-dimensional culture. The azide-modified gelatin was prepared by mixing gelatin with Azide-PEG4-NHS ester so...
Shota Tashiro, Yuta Kusama, Eri Nakatani, Taku Sato, Shinji Sugiura, Toshiyuki Kanamori, Kiyoshi Ohnuma
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016   1683-1684   Jan 2016
An elongated microperfusion culture system was designed to elucidate the effect of soluble factors secreted from differentiated cells on the differentiation of human induced pluripotent stem cells. Our findings were consistent with head-tail patte...
T. Satoh, S. Sugiura, K. Shin, S. Ishida, T. Fujimoto, H. Miyamoto, N. Taneichi, T. Kanamori
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016   519-520   Jan 2016
Here we report a pneumatic pressure-driven multi-throughput multi-organs-on-a-chip system for providing physiological response using human-derived cells in vitro. Since medium circulation in the system was operated by a pneumatic pressure control,...
T. Satoh, G. Narazaki, R. Sugita, H. Kobayashi, S. Sugiura, T. Kanamori
MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences   618-620   Jan 2015
© 15CBMS-0001. Here we report a practically advantageous circulation culture system for an in vitro investigation of vascular endothelial cells under shear stress. A palm-sized culture device equipped with three discrete fluidic circuits in which ...
Fumiki Yanagawa, Masato Tamura, Shinji Sugiura, Toshiyuki Takagi, Kazumi Shin, Kimio Sumaru, Toshiyuki Kanamori
MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences   615-617   Jan 2015
© 15CBMS-0001. This paper reports hydrogels micropatterning for fabrication of 3D perfusable structure using photodegradable hydrogels via click reaction. We also demonstrated that hepatocellular carcinoma cell patterning in the hydrogels can be p...
Shinji Sugiura, Fumiki Yanagawa, Toshiyuki Kanamori
Hyper Bio Assembler for 3D Cellular Systems   203-211   Jan 2015
© Springer Japan 2015. Living cells in human body exhibit their function in microstructured three dimensional (3D) environments composed of soluble factors, extracellular matrix (ECM), and neighboring cells. Fabricating biological 3D in vitro mode...
S. Sugiura, T. Fujimoto, H. Miyamoto, K. Sumaru, K. Shin, F. Yanagawa, N. Taneichi, T. Kanamori
MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences   2101-2102   Jan 2015
© 15CBMS-0001. This paper reports stereolithography technique to fabricate microfluidic devices with optically clear and biocompatible cell culture microchambers. We have successfully demonstrated cell culture for 3 days, phase contrast observatio...
Kimio Sumaru, Toshiyuki Takagi, Shinji Sugiura, Toshiyuki Kanamori
Soft Actuators: Materials, Modeling, Applications, and Future Perspectives   9784431547679 219-229   Jul 2014
© 2014 Springer Japan. All rights reserved. Photoresponsive actuators composed of a hydrogel functionalized with spiropyran are described. The hydrogel exhibits drastic shrinking in rapid response to blue light irradiation in acidic aqueous system...
Fumiki Yanagawa, Takeomi Mizutani, Shinji Sugiura, Kimio Sumaru, Toshiyuki Takagi, Toshiyuki Kanamori
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014   1874-1876   Jan 2014
© 14CBMS. This paper reports a simple technique to synthesize elasticity tunable hybrid hydrogels with an activated-ester-type photocleavable crosslinker (NHS-PC-4armPEG) and non-photocleavable crosslinker (NHS-4armPEG). We demonstrated that synth...
Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014   512-514   Jan 2014
© 14CBMS. This paper reports a novel technique to fabricate perfusable 3D liver tissue in a microfluidic device using photodegradable hydrogels prepared with gelatin. Also, we performed the perfusion culture of human hepatocellular carcinoma (HepG...
Shinji Sugiura, Fumiki Yanagawa, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori, Masato Tamura, Masato Tamura, Hirofumi Matsui
2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014      Jan 2014
© 2014 IEEE. In this work, we present a novel photocleavable crosslinker to form photodegradable hydrogel through one step reaction by mixing with biocompatible polymers containing amino moieties (amino-terminated tetra-arm poly(ethylene glycol) o...
Y. Yamamoto, R. Yamada, K. Hattori, S. Tachikawa, M. Tagaya, T. Sasaki, D. Miyamoto, K. Nakazawa, S. Sugiura, T. Kanamori, K. Ohnuma
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014   901-903   Jan 2014
© 14CBMS. We succeeded in controlling the adhesion of human induced pluripotent stem cells (hiPSCs) on plasma-patterned polydimethylsiloxane (PDMS) by a single-step coating of two ubiquitous proteins, vitronectin and γ-globulin. Our serum- and fee...
Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014   500-502   Jan 2014
© 14CBMS. This paper reports a characterization of cell behavior on photodegradable hydrogels, which were composed of an activated-ester-type photocleavable crosslinker (NHS-PC-4armPEG) and biocompatible polymers containing amino moieties, such as...
R. Yoshimitsu, K. Hattori, S. Sugiura, Y. Kondo, T. Satoh, A. Kurisaki, M. Asashima, K. Ohnuma, T. Kanamori
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   2 1072-1074   Jan 2013
This paper reports on the microfluidic perfusion culture of human induced pluripotent stem cells (hiPSCs) under fully defined culture condition. By screening various ECMs we determined that fibronectin and laminin are appropriate for microfluidic ...
Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Hirofumi Matsui, Toshiyuki Kanamori
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   3 1929-1931   Jan 2013
We present a new methodology for optical cell picking in 3D culture environment, because biological cells often show phenotype alterations by the interactions with pericellular matrix in 3D culture. In this study, we encapsulated cells in photodeg...
Ryotaro Yamada, Koji Hattori, Motohiro Tagaya, Toru Sasaki, Shinji Sugiura, Toshiyuki Kanamori, Kiyoshi Ohnuma, Kiyoshi Ohnuma
2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013      Jan 2013
The goal of this article is to make a pattern of human induced pluripotent stem cells (hiPSCs) on a Polydimethylsiloxane (PDMS) surface. Here, we report that pattering of hiPSCs can be achieved by patterned plasma treatment followed by coating wit...
Taku Satoh, Shinji Sugiura, Kimio Sumaru, Shigenori Ozaki, Shinichi Gomi, Tomoaki Kurakazu, Yasuhiro Oshima, Toshiyuki Kanamori
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   3 1701-1703   Jan 2013
This paper reports a novel cell culture chip, namely "inverting microwell array chip", for cultivation of human induced pluripotent stem cells with controlled microenvironment. Our "inverting microwell array chip" comprises a lower hydrogel microw...
K. Hattori, R. Yoshimitsu, S. Sugiura, A. Maruyama, K. Ohnuma, T. Kanamori
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   3 1448-1450   Jan 2013
This paper presents a simple micropatterning process "masked plasma oxidation" to create a closed microchamber array with an extracellular matrix (ECM). The fabrication process, which consists of homogeneous physical adsorption of ECM and subseque...
F. Yanagawa, S. Sugiura, T. Takagi, K. Sumaru, T. Kanamori
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   1 149-151   Jan 2013
Photodegradable hydrogels have emerged as a powerful platform for studying and directing cell behavior in a spatiotemporally controlled manner. Several research groups have previously reported the formation of photodegradable hydrogels by redox-me...
Shinji Sugiura, Koji Hattori, Yusuke Sakai, Yusuke Sakai, Kohji Nakazawa, Toshiyuki Kanamori
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012   470-472   Jan 2012
This paper reports a microfluidic device composed of a reversibly-assembled microfluidic chamber chip and multi-microwell array chip. This device enabled perfusion culture of spheroid in a microarray format, and detailed post-culture analysis by c...
Shinji Sugiura, Toshiyuki Takagi, Manae Yamaguchi, Kimio Sumaru, Toshiyuki Kanamori
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012   671-673   Jan 2012
Control of cell adhesion and detachment on the micrometer scale is an important issue for understanding cell-substrate or cell-cell interaction. We synthesized a photocleavable crosslinker, which composed of hydrophilic poly(ethylene glycol) (PEG)...
Shinji Sugiura, Shinji Sugiura, Shinji Sugiura, Jae Min Cha, Jae Min Cha, Fumiki Yanagawa, Fumiki Yanagawa, Pinar Zorlutuna, Pinar Zorlutuna, Hojae Bae, Hojae Bae, Ali Khademhosseini, Ali Khademhosseini, Ali Khademhosseini
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012   554-556   Jan 2012
We report the spatiotemporal control of a 3D coculture by combining photocurable and chemically degradable hydrogels for coculture of embryonic stem (ES) cells with HepG2 cells. ES cells were encapsulated in micropatterned photocurable poly(ethyle...
D. Saeki, D. Saeki, S. Sugiura, T. Kanamori, S. Sato, S. Ichikawa
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011   2 762-764   Dec 2011
This paper presents a novel microcompartmentalized reaction system for cell-free protein synthesis using semi-permeable microcapsules. We used a microfluidic device to encapsulate template DNA and enzymes for cell-free protein synthesis into semi-...
T. Yamamoto, D. Saeki, D. Saeki, S. Sugiura, T. Kanamori, S. Sato, S. Ichikawa
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011   3 1585-1587   Dec 2011
This paper reports compartmentalized reaction system applicable to parallelized multi-step biochemical reaction using suspension of the agarose gel microbeads that are chemically modified with multiple affinity ligands. The multistep reaction proc...
S. Sugiura, K. Hattori, T. Kanamori
ECS Transactions   33 197-207   Dec 2010
We report a perfusion culture microchamber array chip for high throughput drug dose response assays. The microchamber array chip was equipped with a serial dilution microfluidic network for generating a logarithmic concentration profile. Cell susp...
K. Hattori, S. Sugiura, T. Kanamori
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010   1 178-180   Dec 2010
We report a microenvironment array: perfusion culture microchamber array with different cell culture conditions. The microenvironment array provides the 4 × 4 combinatorial cell culture conditions composed of four different soluble factors and fou...
Shinji Sugiura, Wataru Imano, Wataru Imano, Toshiyuki Takagi, Kiyotaka Sakai, Toshiyuki Kanamori
Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences   893-895   Jan 2009
We report a high-contrast protein adsorption control method through temperature change on a polydimethylsiloxane (PDMS) microchannel surface using a thermoresponsive polymer, poly(N-isopropylacrylamide) (PNIPAAm). Modification both of protein and ...
Daisuke Saeki, Daisuke Saeki, Daisuke Saeki, Shinji Sugiura, Toshiyuki Kanamori, Seigo Sato, Sosaku Ichikawa
Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences   1428-1430   Jan 2009
This paper reports a novel preparation method of calcium alginate microbeads from water-in-oil-in-water (W/O/W) droplets with ultra-thin oil phase layer. We used the W/O/W droplets as a template of the calcium alginate microbeads and ruptured the ...
Koji Hattori, Shinji Sugiura, Toshiyuki Kanamori
Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences   469-471   Jan 2009
We report a microenvironment array: a perfusion culture microchamber array with controlled microenvironments. A cell culture microenvironment is composed of extracellular matrixes (ECM) as scaffold proteins and growth factors in media. We controll...
Shinji Sugiura, Koji Hattori, Toshiyuki Kanamori
Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences   27-29   Jan 2009
This paper reports a perfusion culture microchamber array chip for high throughput drug dose response assay. The developed microchamber array chip is equipped with a serial dilution microfluidic network for generating concentration profile spannin...
Shinji Sugiura, Jun Ichi Edahiro, Kimio Sumaru, Toshiyuki Takagi, Toshiyuki Kanamori
Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences   460-462   Jan 2009
This paper reports a novel cell micropatterning method in microchannels by means of micropatterned light irradiation of a photoresponsive culture surface. We developed a photoresponsive culture surface on which cell adhering region can be prepared...
Jun Ichi Edahiro, Kimio Sumaru, Shinji Sugiura, Toshiyuki Takagi, Toshiyuki Kanamori
8th World Biomaterials Congress 2008   2 1127   Dec 2008
D. Saeki, D. Saeki, S. Sugiura, T. Baba, T. Kanamori, S. Sato, S. Ichikawa
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference   784-786   Jan 2008
This paper reports a novel microfluidic device to prepare monodisperse water-inoil- in-water (W/O/W) emulsions with an ultra-thin oil phase layer. Our device enabled to prepare the thin-layered W/O/W emulsions in a microchannel with a hydrophobic ...
S. Sugiura, A. Szilágyi, A. Szilágyi, K. Sumaru, T. Takagi, M. Zrínyi, T. Kanamori
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference   838-840   Jan 2008
Novel on-chip fluid control strategies, on-demand formation of arbitrary microchannels and parallel control of multiple microvalves, were successfully demonstrated by PC-controlled maskless micro-patterned light irradiation onto photoresponsive hy...
K. Hattori, S. Sugiura, T. Kanamori
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference   1660-1662   Jan 2008
In a cell-based assay, a serial dilution process is tedious, time-consuming and a source of an experimental error. We designed a serial dilution microfluidic network for efficient cell-based assay on a microchip. The microfluidic network was compo...
András Szilágyi, András Szilágyi, Kimio Sumaru, Shinji Sugiura, Toshiyuki Takagi, Toshiyuki Kanamori, Toshio Shinbo, Miklós Zrínyi
Polymer Preprints, Japan   55 4565-4566   Dec 2006
We prepared novel polymer hydrogel sheets composed of thermo-responsive pNIPAAm main chain and photo-resnposive side chains of spiropyran. The drastic photo-induced dehydration was observed in 0.5 mM HCl aq. at 28°C, and photo-induced micro-relief...
Shinji Sugiura, Kimio Sumaru, Kazuaki Hiroki, Toshiyuki Takagi, Toshiyuki Kanamori
AIChE Annual Meeting, Conference Proceedings      Dec 2006
Shinji Sugiura, Kimio Sumaru, Kazuaki Hiroki, Toshiyuki Takagi, Toshiyuki Kanamori
Polymer Preprints, Japan   55 4567-4568   Dec 2006
Photo-responsive polymer gel, composed of poly(N-isopropylacrylamide) functionalized with spirobenzopyran chromophore (poly-Sp-NIPAAm), is known to shrink in response to blue light irradiation in aqueous solution. In this study, poly-Sp-NIPAAm gel...
Shinji Sugiura, Shinji Sugiura, Tatsuya Oda, Yasuyuki Aoyagi, Ryota Matsuo, Tsuyoshi Enomoto, Kunio Matsumoto, Toshikazu Nakamura, Mitsuo Satake, Atsushi Ochiai, Nobuhiro Ohkohchi, Mitsutoshi Nakajima
AIChE Annual Meeting, Conference Proceedings      Dec 2006
Shinji Sugiura, Aiko Okamura, Tumiko Yoshida, Toshio Shinbo, Toshiyuki Kanamori
AIChE Annual Meeting, Conference Proceedings      Dec 2006
Kimio Sumaru, Shinji Sugiura, Toshiyuki Kanamori
AIChE Annual Meeting, Conference Proceedings      Dec 2006
Kimio Sumaru, Jun Ichi Edahiro, Yuichi Tada, Yuki Ooshima, Shinji Sugiura, Toshiyuki Kanamori, Toshio Shinbo
AIChE Annual Meeting, Conference Proceedings      Dec 2006
Shinji Sugiura, Kimio Sumaru, Katsuhide Ohi, Kazuaki Hiroki, Toshiyuki Takagi, Toshiyuki Kanamori
Polymer Preprints, Japan   55 1713   Oct 2006
Photo-responsive polymer gel, composed of poly(N-isopropylacrylamide) functionalized with spirobenzopyran chromophore (poly-Sp-NIPAAm), is known to shrink in response to blue light irradiation in aqueous solution. In this study, poly-Sp-NIPAAm gel...
Yuichi Tada, Yuichi Tada, Jun Ichi Edahiro, Kimio Sumaru, Toshiyuki Kanamori, Shinji Sugiura, Toshiyuki Takagi, Toshio Shinbo, Yasuo Yoshimi
Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences   966-968   Jan 2006
By using a photo-responsive cell culture surface, which captures the cell strongly in response to light irradiation, we examined a novel layout of micro cell chip, in which living cells were immobilized in a prescribed arrangement in micro channel...
Yuichi Tada, Yuichi Tada, Kimio Sumaru, Shinji Sugiura, Junichi Edahiro, Toshiyuki Takagi, Toshio Shinbo, Toshiyuki Kanamori, Yasuo Yoshimi
Polymer Preprints, Japan   54 4989   Dec 2005
We developed a novel cell chip, which enables us to control cell adhesion of the bottom face in a micro channel by local light irradiation. A flow-type cell chip was constructed by attaching a sheet of poly (dimethyl siloxane) having micro-channel...
Kimio Sumaru, Shinji Sugiura, Toshiyuki Takagi, Katsuhide Ohi, Toshiyuki Kanamori, Toshio Shinbo, Genovéva Filipcsei, Miklós Zrínyi
Polymer Preprints, Japan   54 4682-4683   Dec 2005
We prepared photo-responsive hydrogels composed of N-isopropylacrylamide and acrylate monomer containing a spiropyran residue, and investigated systematically the influence of the condition of cross-linker content and solvent on the photo-responsi...
Yasuhiko Izumida, Yasuhiko Izumida, Shinji Sugiura, Shinji Sugiura, Shinji Sugiura, Tatsuya Oda, Mitsuo Satake, Mitsutoshi Nakajima, Mitsutoshi Nakajima
JAOCS, Journal of the American Oil Chemists' Society   82 73-78   Dec 2005
The development and operation of a modified method for the preparation of quasi-monodisperse (CV < 16%) emulsions are reported. The device uses a microfabricated-nozzle (MN) array, produced by micromachining technology, to achieve a higher rate of...
Shinji Sugiura, Shinji Sugiura, Mitsutoshi Nakajima, Minora Seki
1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings   283-287   Jan 2000
© 2000 IEEE. Microspheres (MS), such as emulsion droplets, multiple emulsions, microparticles, microcapsules, and liposomes bave been utilized in various industries. We proposed a novel microfabricated device for making supermonodispersed emulsion...