論文

査読有り 国際誌
2018年11月30日

Diffracted X-ray Blinking Tracks Single Protein Motions.

Scientific reports
  • Hiroshi Sekiguchi
  • ,
  • Masahiro Kuramochi
  • ,
  • Keigo Ikezaki
  • ,
  • Yu Okamura
  • ,
  • Kazuki Yoshimura
  • ,
  • Ken Matsubara
  • ,
  • Jae-Won Chang
  • ,
  • Noboru Ohta
  • ,
  • Tai Kubo
  • ,
  • Kazuhiro Mio
  • ,
  • Yoshio Suzuki
  • ,
  • Leonard M G Chavas
  • ,
  • Yuji C Sasaki

8
1
開始ページ
17090
終了ページ
17090
記述言語
英語
掲載種別
DOI
10.1038/s41598-018-35468-3

Single molecule dynamics studies have begun to use quantum probes. Single particle analysis using cryo-transmission electron microscopy has dramatically improved the resolution when studying protein structures and is shifting towards molecular motion observations. X-ray free-electron lasers are also being explored as routes for determining single molecule structures of biological entities. Here, we propose a new X-ray single molecule technology that allows observation of molecular internal motion over long time scales, ranging from milliseconds up to 103 seconds. Our method uses both low-dose monochromatic X-rays and nanocrystal labelling technology. During monochromatic X-ray diffraction experiments, the intensity of X-ray diffraction from moving single nanocrystals appears to blink because of Brownian motion in aqueous solutions. X-ray diffraction spots from moving nanocrystals were observed to cycle in and out of the Bragg condition. Consequently, the internal motions of a protein molecule labelled with nanocrystals could be extracted from the time trajectory using this diffracted X-ray blinking (DXB) approach. Finally, we succeeded in distinguishing the degree of fluctuation motions of an individual acetylcholine-binding protein (AChBP) interacting with acetylcholine (ACh) using a laboratory X-ray source.

Web of Science ® 被引用回数 : 4

リンク情報
DOI
https://doi.org/10.1038/s41598-018-35468-3
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30504916
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269541
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000451746700001&DestApp=WOS_CPL
URL
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000451746700001&KeyUID=WOS:000451746700001
URL
http://orcid.org/0000-0002-1655-8231

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