論文

査読有り
2018年4月1日

Lipid environment of membrane proteins in cryo-EM based structural analysis

Biophysical Reviews
  • Kazuhiro Mio
  • ,
  • Chikara Sato

10
2
開始ページ
307
終了ページ
316
記述言語
英語
掲載種別
DOI
10.1007/s12551-017-0371-6
出版者・発行元
Springer Verlag

Cryoelectron microscopy (cryo-EM) in association with a single particle analysis method (SPA) is now a promising tool to determine the structures of proteins and their macromolecular complexes. The development of direct electron detection cameras and image processing technologies has allowed the structures of many important proteins to be solved at near-atomic resolution or, in some cases, at atomic resolution, by overcoming difficulties in crystallization or low yield of protein production. In the case of membrane-integrated proteins, the proteins were traditionally solubilized and stabilized with various kind of detergents. However, the density of detergent micelles diminished the contrast of membrane proteins in cryo-EM studies and made it difficult to obtain high-resolution structures. To improve the resolution of membrane protein structures in cryo-EM studies, major improvements have been made both in sample preparation techniques and in hardware and software developments. The focus of our review is on improvements which have been made in the various techniques for sample preparation for cryo-EM studies, with a specific interest placed on techniques for mimicking the lipid environment of membrane proteins.

リンク情報
DOI
https://doi.org/10.1007/s12551-017-0371-6
URL
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:29256118&KeyUID=MEDLINE:29256118
URL
http://orcid.org/0000-0002-1655-8231

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