2011年12月
Crystallization and preliminary crystallographic analysis of a putative glucokinase/hexokinase from Thermus thermophilus
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
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- 巻
- 67
- 号
- 開始ページ
- 1559
- 終了ページ
- 1562
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1107/S1744309111041145
- 出版者・発行元
- INT UNION CRYSTALLOGRAPHY
Glucokinase/hexokinase catalyzes the phosphorylation of glucose to glucose 6-phosphate, which is the first step of glycolysis. The open reading frame TTHA0299 of the extreme thermophile Thermus thermophilus encodes a putative glucokinase/hexokinase which contains the consensus sequence for proteins from the repressors, open reading frames and sugar kinases family. In this study, the glucokinase/hexokinase from T. thermophilus was purified and crystallized using polyethylene glycol 8000 as a precipitant. Diffraction data were collected and processed to 2.02 angstrom resolution. The crystal belonged to space group P21, with unit-cell parameters a = 70.93, b = 138.14, c = 75.16 angstrom, beta = 95.41 degrees.
- リンク情報
- ID情報
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- DOI : 10.1107/S1744309111041145
- ISSN : 1744-3091
- eISSN : 2053-230X
- Web of Science ID : WOS:000297741100021