2015年6月
Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos
CONSERVATION GENETICS RESOURCES
- ,
- ,
- ,
- 巻
- 7
- 号
- 2
- 開始ページ
- 451
- 終了ページ
- 453
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s12686-014-0393-3
- 出版者・発行元
- SPRINGER
The milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.
- リンク情報
- ID情報
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- DOI : 10.1007/s12686-014-0393-3
- ISSN : 1877-7252
- eISSN : 1877-7260
- Web of Science ID : WOS:000354213300034