論文

査読有り 責任著者
2021年1月1日

Preferable Transplant Site for Hepatocyte Transplantation in a Rat Model

Cell Transplantation
  • Hiroyuki Ogasawara
  • Akiko Inagaki
  • Ibrahim Fathi
  • Takehiro Imura
  • Hiroki Yamana
  • Yoshikatsu Saitoh
  • Muneyuki Matsumura
  • Kengo Fukuoka
  • Shigehito Miyagi
  • Yasuhiro Nakamura
  • Kazuo Ohashi
  • Michiaki Unno
  • Takashi Kamei
  • Masafumi Goto
  • 全て表示

30
開始ページ
096368972110400
終了ページ
096368972110400
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1177/09636897211040012
出版者・発行元
SAGE Publications

Intraportal injection is regarded as the current standard procedure of hepatocyte transplantation (HTx). In islet transplantation, which shares many aspects with HTx, recent studies have clarified that instant blood-mediated inflammatory reaction (IBMIR), characterized by strong innate immune responses, can cause poor engraftment, so other transplant sites to avoid such a reaction have been established. Although IBMIR was reported to occur in HTx, few reports have evaluated alternative transplant sites for HTx. In this study, we sought to determine the optimum transplant site for HTx. Rat hepatocytes (1.0 × 107) were transplanted at the 9 transplant sites (intraportal (IPO), intrasplenic (IS), liver parenchyma, subcutaneous, intraperitoneal, renal subcapsular, muscle, inguinal subcutaneous white adipose tissue, and omentum) of analbuminemic rats. The serum albumin levels, immunohistochemical staining (albumin, TUNEL, and BrdU), and in vivo imaging of the grafts were evaluated. The serum albumin levels of the IPO group were significantly higher than those of the other groups ( p < .0001). The BrdU-positive hepatocyte ratio of liver in the IS group (0.9% ± 0.2%) was comparable to that of the IPO group (0.9% ± 0.3%) and tended to be higher than that of the spleen in the IS group (0.5% ± 0.1%, p = .16). Considering the in vivo imaging evaluation and the influence of splenectomy, the graft function in the IS group may be almost entirely achieved by hepatocytes that have migrated to the liver. The present study clearly showed that the intraportal injection procedure is more efficient than other procedures for performing HTx

リンク情報
DOI
https://doi.org/10.1177/09636897211040012
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000698673800001&DestApp=WOS_CPL
URL
http://journals.sagepub.com/doi/pdf/10.1177/09636897211040012
URL
http://journals.sagepub.com/doi/full-xml/10.1177/09636897211040012
ID情報
  • DOI : 10.1177/09636897211040012
  • ISSN : 0963-6897
  • eISSN : 1555-3892
  • Web of Science ID : WOS:000698673800001

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