J-GLOBAL         Last updated: Oct 16, 2019 at 12:26
Nippon Veterinary and Life Science University
Faculty of Veterinary Science, School of Veterinary Medicine, Department of Veterinary Pathology
Job title

Research Areas


Academic & Professional Experience

Professor, School of Veterinary Medinine, Nippon Veterinary and Life-science University (NVLU)
Associate professor, School of Veterinary Medinine, Nippon Veterinary and Life-science University (NVLU)
Senior assistant professor, School of Veterinary Medinine, Nippon Veterinary and Life-science University (NVLU)
Assistant professor (research associate), School of Medinine, Keio University
Assistant professor (research associate), School of Medinine, Nagasaki University
Research resident, National Insititute for Longevity Sciences


Faculty of Veterinary Medicine and Animal Sciences, Nippon Veterinary and Animal Science University
Graduate School, Division of Medicine, Nagoya University

Published Papers

Ohkusu-Tsukada K, Yamashita T, Tsukada T, Takahashi K
Genes and immunity   20(1) 74-81   Jan 2019   [Refereed]
Environmental factors and the major histocompatibility complex (MHC) are involved in the pathogenesis of atopic dermatitis (AD). However, MHC type (H2 haplotype) of AD model mice NC/Nga is poorly understood. Alloreactive CD8+ or CD4+ T cells in NC...
Ohkusu-Tsukada K. Ito D. Okuno Y. Tsukada T. Takahashi K.
Scientific Reports   8 2586   Feb 2018   [Refereed]
We recently advocated in favour of naming a novel H2-haplotype consisting of Kd, D/Ldm7, I-Ak and I-Ek in the atopic dermatitis (AD) mouse model NC/Nga as “H-2nc.” The role of the H2-haplotype in AD development was investigated in H2b-congenic NC/...
Ohkusu-Tsukada K. Ito D. Takahashi K.
International Archives of Allergy and Immunology   176(2) 91-100   2018   [Refereed]
Background: Although immunosuppressants for therapy of atopic dermatitis (AD) are still being sought, proteasome inhibitors are also potential candidates for the treatment of AD. Proteasome inhibitors exert various effects by blocking proteasomal ...
Ohkusu-Tsukada K, Tsukada T, Takahashi K
Bioscience, biotechnology, and biochemistry   81(11) 2160-2163   Nov 2017   [Refereed]
Superantigens (SAgs) are powerful T-cell stimulatory proteins. Because an atopic dermatitis (AD) model NC/Nga mice had two endogenous SAgs, namely minor lymphocyte-stimulating locus-1a (Mls-1a) and mouse mammary tumor virus (MMTV)(SHN), SAg-respon...
Kazuki Kishida, Kozo Ohkusu-Tsukada, Makito Hori, Masaki Konnai, Chieko Abiko, Yoshikazu Suzuki, Yukito Yamanome, Hisashi Yoshimura, Masaki Michishita, Kimimasa Takahashi
Veterinary Journal   196(2) 160-166   May 2013   [Refereed]
Intrahepatic eosinophilic proliferative pylephlebitis (EPP) in Japanese Black (JB) cattle generally has been considered to be an atypical form of fascioliasis. However, there are many cases of EPP in which no Fasciola spp. have been detected in th...
Kozo Ohkusu-Tsukada, Shigeki Ohta, Shigeki Ohta, Yutaka Kawakami, Masahiro Toda, Masahiro Toda
International Journal of Cancer   128(1) 119-131   Jan 2011   [Refereed]
Use of adequate adjuvant is necessary for induction of effective antitumor immune responses. To develop an effective adjuvant for cancer immunotherapy, we selected formalin-inactivated (f)-HSV as an adjuvant component, and analyzed the mechanisms ...
Kozo Ohkusu-Tsukada, Masahiro Toda, Heiichiro Udono, Yutaka Kawakami, Kimimasa Takahashi
European Journal of Immunology   40(4) 1011-1021   Apr 2010   [Refereed]
Cancer-induced immunotolerance mediated by inducible Treg (iTreg) is a major obstacle to cancer immunotherapy. In a basic study of immunotolerance, injection of an endogenous superantigen, i.e. the minor lymphocyte stimulatory (Mls)-1a, into speci...
Kozo Ohkusu-Tsukada, Norio Tominaga, Norio Tominaga, Heiichiro Udono, Heiichiro Udono, Katsuyuki Yui, Katsuyuki Yui
Molecular and Cellular Biology   24(16) 6957-6966   Aug 2004   [Refereed]
In anergic T cells, T-cell receptor (TCR)-mediated responses are functionally inactivated by negative regulatory signals whose mechanisms are poorly understood. Here, we show that CD4+ T cells anergized in vivo by superantigen Mls-1a express a sca...
Kozo Ohkusu-Tsukada, Kozo Ohkusu-Tsukada, Teruyo Tsukada, Teruyo Tsukada, Ken Ichi Isobe, Ken Ichi Isobe
Journal of Immunology   163(4) 1966-1972   Aug 1999   [Refereed]
Development and aging of the immune system lead to an accumulation of memory T cells over the long term. The predominance of T cells of the memory phenotype in the T cell population induces an age-related decline in protective immune responses. We...
Ohkusu K, Du J, Isobe KI, Yi H, Akhand AA, Kato M, Suzuki H, Hidaka H, Nakashima I.
Journal of Immunology   159(5) 2082-2084   1997   [Refereed]
In CD2/protein kinase C alpha (PKC alpha)-overexpressing human CD2/rabbit PKC alpha transgenic mice, an aging-dependent increase in PKC alpha expression and a decrease in proliferative responsiveness of splenic T cells were promoted. We found that...
Kozo Ohkusu, Ken Ichi Isobe, Hiroyoshi Hidaka, Izumi Nakashima, Izumi Nakashima
European Journal of Immunology   25(11) 3180-3186   Nov 1995   [Refereed]
MRL-lpr mice are severely impaired in the Fas pathway of apoptosis induction. We here evaluate another pathway of apoptosis induction in MRL-lpr mice which is protein kinase C (PKC) dependent. Despite the defect of the Fas pathway, apoptosis devel...


Machida Y, Michishita M, Wada M, Hori T, Kawakami E, Yoshimura H, Ohkusu-Tsukada K, Taya K, Takahashi K
Journal of comparative pathology   156(2-3) 178-182   Feb 2017   [Refereed]
Nakahira R, Yoshida R, Michishita M, Ohkusu-Tsukada K, Takahashi K
Comparative medicine   66(1) 25-29   Feb 2016   [Refereed]
Ganglion cell-like (GL) cells reside in the dermis of the ventral skin of mature male Djungarian hamsters (Phodopus sugorus) and express androgen receptor (AR). To assess whether GL cells have androgen-dependent behavior, we evaluated the histolog...
Arai K, Kobayashi M, Harada Y, Hara Y, Michishita M, Ohkusu-Tsukada K, Takahashi K
American journal of veterinary research   77(1) 77-83   Jan 2016   [Refereed]
Yoshimura H, Kimura-Tsukada N, Ono Y, Michishita M, Ohkusu-Tsukada K, Matsuda Y, Ishiwata T, Takahashi K
Veterinary pathology   52(6) 1227-1234   Nov 2015   [Refereed]
Kishimoto TE, Yoshimura H, Saito N, Michishita M, Kanno N, Ohkusu-Tsukada K, Takahashi K
Journal of comparative pathology   153(2-3) 111-115   Aug 2015   [Refereed]
Yoshimura H, Michishita M, Ohkusu-Tsukada K, Matsuda Y, Ishiwata T, Naito Z, Takahashi K
Veterinary pathology   52(1) 92-96   Jan 2015   [Refereed]
Yoshimura H, Nakahira R, Kishimoto TE, Michishita M, Ohkusu-Tsukada K, Takahashi K
Veterinary pathology   51(6) 1090-1095   Nov 2014   [Refereed]
Yoshimura H, Matsuda Y, Kawamoto Y, Michishita M, Ohkusu-Tsukada K, Takahashi K, Naito Z, Ishiwata T
Journal of comparative pathology   149(2-3) 225-228   Aug 2013   [Refereed]
Masaki Michishita, Rui Akiyoshi, Hisashi Yoshimura, Takuo Katsumoto, Hitoshi Ichikawa, Kozo Ohkusu-Tsukada, Takayuki Nakagawa, Nobuo Sasaki, Kimimasa Takahashi
Research in Veterinary Science   91(2) 254-260   Oct 2011   [Refereed]
There is increasing evidence for the presence of cancer stem cells in several solid tumors, and these cancer stem cells have a potential role in tumor initiation, aggression, and recurrence. The stem cell-like properties of spheres derived from ca...
Machida Y, Yoshimura H, Nakahira R, Michishita M, Ohkusu-Tsukada K, Takahashi K
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc   23(4) 840-844   Jul 2011   [Refereed]
Hisashi Yoshimura, Masaki Michishita, Kozo Ohkusu-Tsukada, Kimimasa Takahashi
Histology and Histopathology   26(3) 297-305   Mar 2011   [Refereed]
Myofibroblasts and extracellular matrix protein tenascin-C (Tn-C) are known to be implicated in cancer progression in human cancer. In feline mammary tumors that are a suitable model for human breast cancer, however, little is known about stromal ...
H. Yoshimura, M. Michishita, K. Ohkusu-Tsukada, K. Takahashi
Veterinary Pathology   48 313-321   Jan 2011   [Refereed]
The aims of this study were to determine whether the appearance of stromal myofibroblasts and the expression of tenascin-C (Tn-C) correlate with the grade of malignancy in canine mammary tumors and to determine the main cellular source of Tn-C in ...
Michishita M, Momozawa Y, Oizumi T, Ohkusu-Tsukada K, Takahashi K.
Vet. Dermatol.   21 408-411   2010   [Refereed]
Ueda R, Ohkusu-Tsukada K, Fusaki N, Soeda A, Kawase T, Kawakami Y, Toda M.
Int. J. Cancer   126(4) 919-929   2010   [Refereed]
Yoshimura H, Kimura N, Michishita M, Ohkusu-Tsukada K, Takahashi K
J Vet Diagn Invest.   22(2) 305-309   2010
A lipid-rich carcinoma of the mammary gland was diagnosed in a female Djungarian hamster (Phodopus sungorus), which was kept as an indoor pet. The animal underwent surgery for a primary tumor arising in the mammary gland at the age of 16 months, a...
Yaguchi M, Tabuse M, Ohta S, Ohkusu-Tsukada K, Takeuchi T, Yamane J, Katoh H, Nakamura M, Matsuzaki Y, Yamada M, Itoh T, Nomura T, Toyama Y, Okano H, Toda M.
Neurosci. Res.   65(4) 384-392   2009   [Refereed]
Tominaga N, Ohkusu-Tsukada K, Udono H, Abe R, Matsuyama T, Yui K.
International Immunology   15(1) 1-10   Jan 2003   [Refereed]
Nakashima I, Du J, Yokoyama T, Kawamoto Y, Ohkusu-Tsukada K, Isobe K-I.
Current Genomics   4(5) 435-443   2003   [Refereed]
Xiao H, Hasegawa T, Miyaishi O, Ohkusu K, Isobe K.
Biochem. Biophys. Res. Commun.   237(2) 457-460   1997   [Refereed]
Ma L, Pu MY, Yi H, Akhand AA, Ohata N, Ohkusu K, Kato M, Iwamoto T, Isobe K, Hamaguchi M, Nakashima I.
J. Cell. Biochem.   59(1) 33-41   1995   [Refereed]
Pu M, Ma L, Ohkusu K, Isobe K, Taguchi R, Ikezawa H, Hamaguchi M, Nakashima I.
FEBS Lett.   361(2/3) 295-298   1995   [Refereed]
Rahman SM, Pu MY, Yi H, Ohkusu K, Kato M, Isobe K, Taguchi R, Ikezawa H, Nakashima I.
Infect. Immun.   63(1) 259-263   1995   [Refereed]
Ichihara M, Iwamoto T, Isobe K, Takahashi M, Nakayama A, Pu M, Dai Y, Parashar A, Ohkus K, Kato M, Hotta T, Nakashima I.
Br. J. Cancer   71(4) 808-813   1995   [Refereed]
Iwamoto T, Ohkusu K, Watanabe M, Hidaka H, Nakashima I.
J. Cell. Biochem.   55(2) 264-271   1994   [Refereed]
Nakashima I, Pu MY, Nishizaki A, Rosila I, Ma L, Katano Y, Ohkusu K, Rahman SM, Isobe K, Hamaguchi M
J. Immunol.   152(3) 1064-1070   1994   [Refereed]
Cross-linking with specific ligand is a general requirement for ordered activation of cell surface receptors. In this study we demonstrated a novel pathway for disregulated receptor activation through a redox mechanism. Treatment of murine thymocy...
Iwamoto T, Taniguchi M, Asai N, Ohkusu K, Nakashima I, Takahashi M.
Oncogene   8(4) 1087-1091   1993   [Refereed]
Nakashima I, Pu MY, Hamaguchi M, Iwamoto T, Rahman SM, Zhang YH, Kato M, Ohkusu K, Katano Y, Yoshida T.
J. Immunol.   151(7) 3511-3520   1993   [Refereed]
The signal delivery pathway triggered by crosslinking CD3 and Thy-1 together (CD3/Thy-1 crosslinkage) on murine thymocytes for cellular DNA fragmentation/growth inhibition was analyzed. The treatment of thymocytes with herbimycin A as a specific t...

Research Grants & Projects

Research and development for practical use of individualized canine cancer immunotherapy
Japan Science and Technology Agency: Grant-in-Aid for Scientific Research (B)
Project Year: Apr 2019 - Mar 2023    Investigator(s): OHKUSU-TSUKADA Kozo
The establishment of DLA-88 typing for cancer peptide vaccine in dog
Japan Science and Technology Agency: Grant-in-Aid for Scientific Research
Project Year: Apr 2014 - Mar 2017    Investigator(s): OHKUSU-TSUKADA Kozo
Research on the role of p38 MAP kinase in the maintenance of T cells anagy
Japan Science and Technology Agency: Grant-in-Aid for Scientific Research
Project Year: Apr 2000 - Mar 2002    Investigator(s): OHKUSU-TSUKADA Kozo
Research to overcome the immunotolerance in tumor immunity
Research of mechanism of stress induced inflammatory autoimmune disease