論文

査読有り
2007年12月

PtdIns(3,4,5)P3 regulates spindle orientation in adherent cells

DEVELOPMENTAL CELL
  • Fumiko Toyoshima
  • ,
  • Shigeru Matsurnura
  • ,
  • Hiroko Morimoto
  • ,
  • Masaru Mitsushima
  • ,
  • Eisuke Nishida

13
6
開始ページ
796
終了ページ
811
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.devcel.2007.10.014
出版者・発行元
CELL PRESS

Cultured adherent cells divide on the substratum, leading to formation of the cell monolayer. However, how the orientation of this anchorage-dependent cell division is regulated remains unknown. We have previously shown that integrin-dependent adhesion orients the spindle parallel to the substratum, which ensures this anchorage-dependent cell division. Here, we show that phosphatidylinositol-3,4,5-triphosphate (PtdIns(3,4,5)P3) is essential for this spindle orientation control. In metaphase, PtdIns(3,4,5)P3 is accumulated in the midcortex in an integrin-dependent manner. Inhibition of phosphatidylinositol-3-OH kinase (PI(3)K) reduces the accumulation of PtdIns(3,4,5)P3 and induces spindle misorientation. Introduction of PtdIns(3,4,5)P3 to these cells restores the midcortical accumulation of PtdIns(3,4,5)P3 and proper spindle orientation. PI(3)K inhibition causes dynein-dependent spindle rotations along the z-axis, resulting in spindle misorientation. Moreover, dynactin, a dynein-binding partner, is accumulated in the midcortex in a PtdIns(3,4,5)P3-dependent manner. We propose that PtdIns(3,4,5)P3 directs dynein/dynactin-dependent pulling forces on spindles to the midcortex, and thereby orients the spindle parallel to the substratum.

リンク情報
DOI
https://doi.org/10.1016/j.devcel.2007.10.014
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902206509001277
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18061563
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000251488100010&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.devcel.2007.10.014
  • ISSN : 1534-5807
  • J-Global ID : 200902206509001277
  • PubMed ID : 18061563
  • Web of Science ID : WOS:000251488100010

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