J-GLOBAL         Last updated: Nov 6, 2018 at 11:56
The University of Tokyo
Graduate School of Arts and Sciences
Job title

Research Areas


Published Papers

Shou Soeda, Shou Soeda, Kaori Yamada-Nomoto, Kaori Yamada-Nomoto, Tatsuo Michiue, Miho Ohsugi
Developmental Cell   47 363-376   Nov 2018   [Refereed]
© 2018 Elsevier Inc. During vertebrate fertilization, sperm chromatin remodeling occurs concomitantly with maternal chromosome segregation at anaphase II, leading to simultaneous formation of two pronuclei. In mammals, these processes take much lo...
Keishi Shintomi, Fukashi Inoue, Fukashi Inoue, Hiroshi Watanabe, Keita Ohsumi, Miho Ohsugi, Tatsuya Hirano
Science   356 1284-1287   Jun 2017   [Refereed]
© The Authors, some rights reserved. The nucleosome is the fundamental structural unit of eukaryotic chromatin. During mitosis, duplicated nucleosome fibers are organized into a pair of rod-shaped structures (chromatids) within a mitotic chromosom...
Tsubasa Ohashi, Tadashi Yamamoto, Tadashi Yamamoto, Yuji Yamanashi, Miho Ohsugi
FEBS Letters   590 1053-1063   Apr 2016   [Refereed]
© 2016 Federation of European Biochemical Societies. In mammals, γ-tubulin, a key protein in microtubule nucleation, is encoded by two genes, TUBG1 and TUBG2. Human TUBG1 and TUBG2 mRNA are expressed ubiquitously and predominantly in preimplantati...
Shou Soeda, Kaori Yamada-Nomoto, Kaori Yamada-Nomoto, Miho Ohsugi, Miho Ohsugi, Miho Ohsugi
Journal of Cell Science   129 3609-3619   Jan 2016   [Refereed]
© 2016. Published by The Company of Biologists Ltd. Mitotic chromosomes move dynamically along the spindle microtubules using the forces generated by motor proteins such as chromokinesin Kid (also known as KIF22). Kid generates a polar ejection fo...
Sung Joon Park, Katsuhiko Shirahige, Miho Ohsugi, Kenta Nakai
Nucleic Acids Research   43    Jan 2015   [Refereed]
© The Author(s) 2014. DBTMEE (http://dbtmee.hgc.jp/) is a searchable and browsable database designed to manipulate gene expression information from our ultralarge-scale whole-transcriptome analysis of mouse early embryos. Since integrative approac...
Sung Joon Park, Makiko Komata, Fukashi Inoue, Kaori Yamada, Kenta Nakai, Miho Ohsugi, Katsuhiko Shirahige
Genes and Development   27 2736-2748   Dec 2013   [Refereed]
Fertilization precisely choreographs parental genomes by using gamete-derived cellular factors and activating genome regulatory programs. However, the mechanism remains elusive owing to the technical difficulties of preparing large numbers of high...
Shou Soeda, Kaori Yamada, Miho Ohsugi, Miho Ohsugi, Miho Ohsugi
Genes to Cells   18 850-858   Oct 2013   [Refereed]
Mammalian oocytes are arrested at metaphase II due to high MAP kinase activity. After fertilization, oocytes resume meiosis, leading to female chromosome segregation, polar body emission and pronuclear (PN) formation. Previous biochemical studies ...
Yuko Ozaki, Hirotaka Matsui, Hiroya Asou, Akiko Nagamachi, Daisuke Aki, Hiroaki Honda, Shin'ichiro Yasunaga, Yoshihiro Takihara, Tadashi Yamamoto, Tadashi Yamamoto, Shunsuke Izumi, Miho Ohsugi, Toshiya Inaba
Molecular Cell   47 694-706   Sep 2012   [Refereed]
During prometaphase, dense microtubule nucleation sites at centrosomes form robust spindles that align chromosomes promptly. Failure of centrosome maturation leaves chromosomes scattered, as seen routinely in cancer cells, including myelodysplasti...
Akinori Takahashi, Chisato Kikuguchi, Masahiro Morita, Tetsuhiro Shimodaira, Noriko Tokai-Nishizumi, Kazumasa Yokoyama, Miho Ohsugi, Toru Suzuki, Tadashi Yamamoto, Tadashi Yamamoto
Biochemical and Biophysical Research Communications   419 268-273   Mar 2012   [Refereed]
The stability of mRNA influences the dynamics of gene expression. The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and contributes to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal ro...
Natsuko Masuda, Tetsuhiro Shimodaira, Shu Jen Shiu, Noriko Tokai-Nishizumi, Tadashi Yamamoto, Miho Ohsugi
Cell structure and function   36 261-267   Dec 2011   [Refereed]
The precise control of spindle microtubule (MT) dynamics is essential for chromosome capture and alignment. Kif18A/kinesin-8, an essential regulator of kinetochore MT dynamics, accumulates at its plus-ends in metaphase but not prometaphase cells. ...
Tomoya S. Kitajima, Miho Ohsugi, Jan Ellenberg
Cell   146 1042   Sep 2011   [Refereed]
Tomoya S. Kitajima, Miho Ohsugi, Miho Ohsugi, Jan Ellenberg
Cell   146 568-581   Aug 2011   [Refereed]
Chromosomes must establish stable biorientation prior to anaphase to achieve faithful segregation during cell division. The detailed process by which chromosomes are bioriented and how biorientation is coordinated with spindle assembly and chromos...
Naoki Oshimori, Naoki Oshimori, Xue Li, Miho Ohsugi, Tadashi Yamamoto
EMBO Journal   28 2066-2076   Jul 2009   [Refereed]
Microtubule-nucleation activity and structural integrity of the centrosome are critical for various cellular functions. The γ-tubulin ring complexes (γTuRCs) localizing to the pericentriolar matrix (PCM) of the centrosome are major sites of microt...
Miho Ohsugi, Kenjiro Adachi, Reiko Horai, Shigeru Kakuta, Katsuko Sudo, Hayato Kotaki, Noriko Tokai-Nishizumi, Hiroshi Sagara, Yoichiro Iwakura, Tadashi Yamamoto
Cell   132 771-782   Mar 2008   [Refereed]
Toward the end of mitosis, neighboring chromosomes gather closely to form a compact cluster. This is important for reassembling the nuclear envelope around the entire chromosome mass but not individual chromosomes. By analyzing mice and cultured c...
Kiyoshi Tahara, Masatoshi Takagi, Miho Ohsugi, Takefumi Sone, Fumiko Nishiumi, Kazuhiro Maeshima, Yasuomi Horiuchi, Noriko Tokai-Nishizumi, Fumio Imamoto, Tadashi Yamamoto, Shingo Kose, Naoko Imamoto
Journal of Cell Biology   180 493-506   Feb 2008   [Refereed]
Nucleocytoplasmic transport factors mediate various cellular processes, including nuclear transport, spindle assembly, and nuclear envelope/pore formation. In this paper, we identify the chromokinesin human kinesin-like DNA binding protein (hKid) ...
K. Haraguchi, M. Ohsugi, Y. Abe, Y. Abe, K. Semba, T. Akiyama, T. Yamamoto, T. Yamamoto
Oncogene   27 274-284   Jan 2008   [Refereed]
The Wnt signaling pathway is essential for embryonic development and carcinogenesis. Upon Wnt stimulation, β-catenin is stabilized and associates with T-cell factor or lymphoid enhancing factor, thereby activating transcription of target genes. In...
Downregulation of a candidate myeloid tumor suppressor gene isolated from 7q21 induces abnormal mitosis due to insufficient centrosome maturation.
Mott Hiroya, Matsui Hirotaka, Ozaki Yuko, Nagamachi Akiko, Aki Daisuke, Ohsugi Miho, Inaba Toshiya
BLOOD   110(11) 776A   Nov 2007   [Refereed]
Shin Ichiro Kina, Tohru Tezuka, Shinji Kusakawa, Yasushi Kishimoto, Yasushi Kishimoto, Yasushi Kishimoto, Sho Kakizawa, Koichi Hashimoto, Koichi Hashimoto, Miho Ohsugi, Yuji Kiyama, Reiko Horai, Katsuko Sudo, Katsuko Sudo, Shigeru Kakuta, Yoichiro Iwakura, Masamitsu Iino, Masanobu Kano, Masanobu Kano, Toshiya Manabe, Toshiya Manabe, Tadashi Yamamoto
European Journal of Neuroscience   26 2269-2278   Oct 2007   [Refereed]
Although protein-tyrosine phosphorylation is important for hippocampus-dependent learning, its role in cerebellum-dependent learning remains unclear. We previously found that PTPMEG, a cytoplasmic protein-tyrosine phosphatase expressed in Purkinje...
Chen Yu Bai, Miho Ohsugi, Yoshinori Abe, Tadashi Yamamoto
Journal of Cell Science   120 2828-2837   Aug 2007   [Refereed]
Focal adhesion protein ZRP-1/TRIP6 has been implicated in actin reorganization and cell motility. The role of ZRP-1, however, remained obscure because previously reported data are often conflicting one another. In the present study, we examined ro...
Abnormal mitosis and genetic instability induced by downregulation of candidate myeloid tumor suppressor genes isolated from the long arm of chromosome 7.
Asou Hiroya, Matsui Hirotaka, Ozaki Yuko, Takemura Toshiyuki, Nagamachi Akiko, Daisuke Aki, Ohsugi Miho, Inaba Toshiya
BLOOD   108(11) 120A   Nov 2006   [Refereed]
Naoki Oshimori, Miho Ohsugi, Tadashi Yamamoto
Nature Cell Biology   8 1095-1101   Oct 2006   [Refereed]
Formation of a bipolar spindle is essential for faithful chromosome segregation at mitosis. Because centrosomes define spindle poles, defects in centrosome number and structural organization can lead to a loss of bipolarity. In addition, microtubu...
Yoshinori Abe, Yoshinori Abe, Miho Ohsugi, Keiko Haraguchi, Jiro Fujimoto, Tadashi Yamamoto
FEBS Letters   580 782-788   Feb 2006   [Refereed]
LATS2 is a human homolog of Drosophila tumor suppressor lats/warts, and encodes a mitotic kinase whose physiological roles remain to be elucidated. We performed yeast two-hybrid screening and identified a LIM protein Ajuba, as a binding partner of...
Noriko Tokai-Nishizumi, Miho Ohsugi, Emiko Suzuki, Tadashi Yamamoto
Molecular Biology of the Cell   16 5455-5463   Nov 2005   [Refereed]
The human chromokinesin Kid/kinesin-10, a plus end-directed microtubule (MT)-based motor with both microtubule- and DNA-binding domains, is required for proper chromosome alignment at the metaphase plate. Here, we performed RNA interference experi...
Tomoya S. Kitajima, Silke Hauf, Miho Ohsugi, Tadashi Yamamoto, Yoshinori Watanabe, Yoshinori Watanabe
Current Biology   15 353-359   Feb 2005   [Refereed]
Shugoshin (Sgo) proteins constitute a conserved protein family defined as centromeric protectors of Rec8-containing cohesin complexes in meiosis [1-4]. In vertebrate mitosis, Scc1/Rad21-containing cohesin complexes are also protected at centromere...
Katsuyuki Shiroguchi, Katsuyuki Shiroguchi, Miho Ohsugi, Masaki Edamatsu, Tadashi Yamamoto, Yoko Y. Toyoshima
Journal of Biological Chemistry   278 22460-22465   Jun 2003   [Refereed]
Chromokinesin Kid (kinesin-like DNA-binding protein) localizes on spindles and chromosomes and has important roles in generating polar ejection force on microtubules in the metaphase. To understand these functions of Kid at the molecular level, we...
Miho Ohsugi, Noriko Tokai-Nishizumi, Katsuyuki Shiroguchi, Yoko Y. Toyoshima, Jun Ichiro Inoue, Tadashi Yamamoto
EMBO Journal   22 2091-2103   May 2003   [Refereed]
The chromokinesin Kid is important in chromosome alignment at the metaphase plate. Here, we report that Kid function is regulated by phosphorylation. We identify Ser427 and Thr463 as M phase-specific phosphorylation sites and Cdc2-cyclin B as a Th...
Rieko Ajima, Naoko Ikematsu, Miho Ohsugi, Yutaka Yoshida, Tadashi Yamamoto
Gene   253 215-220   Aug 2000   [Refereed]
Human Tob2 is a member of the Tob/BTG1 anti-proliferative family of proteins. Here, we report the molecular cloning and characterization of the mouse tob2 gene. The tob2 gene contains an open reading frame of 345 amino acids with an 89% identity t...
Naoko Ikematsu, Yutaka Yoshida, Junko Kawamura-Tsuzuku, Miho Ohsugi, Masanori Onda, Momoki Hirai, Jiro Fujimoto, Tadashi Yamamoto
Oncogene   18 7432-7441   Dec 1999   [Refereed]
Human cDNAs encoding a novel member of Tob/BTG1 anti-proliferative family proteins were cloned. The putative protein product termed Tob2 consisted of 344 amino acids with high similarity to the Tob protein. The tob2 mRNA was 4.1 kb long and was ub...
Emiko Osaki, Yukio Nishina, Johji Inazawa, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Miho Ohsugi, Tohru Tezuka, Mitsuaki Yoshida, Kentaro Semba
Nucleic Acids Research   27 2503-2510   Jun 1999   [Refereed]
Sox family proteins are characterized by a unique DNA-binding domain, a HMG box which shows at least 50% sequence similarity with mouse Sry, the sex-determining factor. At present almost 30 Sox genes have been identified. Members of this family ha...
Miho Ohsugi, Satomi Kuramochi, Satoru Matsuda, Tadashi Yamamoto
Journal of Biological Chemistry   272 33092-33099   Dec 1997   [Refereed]
We identified a novel gene encoding protein-tyrosine phosphatase using a polymerase chain reaction-based method. Northern blot hybridization of RNAs from various tissues with the polymerase chain reaction-amplified DNA fragment showed that this ge...
Satoru Matsuda, Junko Kawamura-Tsuzuku, Miho Ohsugi, Michihiro Yoshida, Mitsuru Emi, Yusuke Nakamura, Masanori Onda, Yutaka Yoshida, Akiko Nishiyama, Tadashi Yamamoto
Oncogene   12 705-713   Dec 1996   [Refereed]
We have molecularly cloned a cDNA for a novel protein termed Tob (Transducer of ErbB-2) that interacts with the c-erbB-2 gene product p185 erbB2 . Nucleotide sequencing reveals that the Tob protein is a 45 kDa protein that does not contain either ...
Junko Kanoh, Yoshinori Watanabe, Miho Ohsugi, Miho Ohsugi, Yuichi Iino, Masayuki Yamamoto, Masayuki Yamamoto
Genes to Cells   1 391-408   Dec 1996   [Refereed]
Background: Fission yeast cells arrest at G1 phase when starved of nitrogen. The molecular mechanism that ensures this arrest is poorly understood. We took a genetic approach to this problem. Results: The fission yeast gad7-1 mutant failed to arre...
Satomi Kuramochi, Satoru Matsuda, Yoichi Matsuda, Toshiyuki Saitoh, Miho Ohsugi, Tadashi Yamamoto
FEBS Letters   378 7-14   Jan 1996   [Refereed]
Novel murine cDNAs encoding a receptor-like protein tyrosine phosphatase, termed Byp (HPTP beta-like tyrosine phosphatase) were cloned. The putative Byp protein consists of 1238 amino acids, which possesses a single catalytic domain in the cytopla...


Miho Ohsugi, Naoki Oshimori
Seikagaku   80 210-214   Dec 2008   [Refereed]
Pronuclear size control in mouse zygotes
Ohsugi Miho, Watanabe Hiroshi
GENES & GENETIC SYSTEMS   91(6) 334   Dec 2016   [Refereed]
Miho Ohsugi, Naoki Oshimori, Tadashi Yamamoto
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   52 880-885   Jul 2007   [Refereed]

Research Grants & Projects

Mitotic spindle assembly and chromosome segregation
Maternally controlled early embryonic cell divisions and their involvement in mammalian development
JST Basic Research Programs (Precursory Research for Embryonic Science and Technology :PRESTO)
Project Year: 2008 - 2011