論文

査読有り
2019年11月

Hypervirulent clade 2, ribotype 019/sequence type 67 Clostridioides difficile strain from Japan.

Gut Pathog
  • Ryoichi Saito
  • ,
  • Yukino Usui
  • ,
  • Alafate Ayibieke
  • ,
  • Jun Nakajima
  • ,
  • Isaac Prah
  • ,
  • Kazunari Sonobe
  • ,
  • Yoshibumi Aiso
  • ,
  • Shiori Ito
  • ,
  • Yasuhiro Itsui
  • ,
  • Yoshiro Hadano
  • ,
  • Yoko Nukui
  • ,
  • Ryuji Koike
  • ,
  • Shuji Tohda

11
開始ページ
54
終了ページ
54
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/s13099-019-0336-3

Clostridioides difficile ribotype (RT) 019/sequence type (ST) 67 strains belong to a hypervirulent lineage closely related to RT027/ST1; however, limited data are available for hypervirulent clade 2 lineages in Japan. Herein, we report the draft genome of a C. difficile strain B18-123 belonging to clade 2, RT019/ST67 for the first time in Japan.<br />
<br />
RESULTS:<br />
The pathogenicity locus carried by B18-123 (19.6 kb) showed higher homology (97.29% nucleotide identity) with strain R20291 (RT027/ST1) than the reference strain 630 (RT012/ST54), and B18-123 harbored 8-nucleotide substitutions in tcdC. However, it did not contain an 18-base pair (bp) deletion or a single-bp deletion at position 117 in tcdC, which was identified in the previous strain R20291. A cytotoxicity assay revealed similar cytotoxicity levels between strains B18-123 and ATCC BAA-1870 (RT027/ST1). The B18-123 strain was found to be susceptible to metronidazole and vancomycin.<br />
<br />
CONCLUSION:<br />
Our findings contribute to the further understanding of the characteristics of hypervirulent clade 2 including RT019/ST67 lineages.

リンク情報
DOI
https://doi.org/10.1186/s13099-019-0336-3
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31700548