論文

査読有り
2019年9月

Whole-genome analysis of EC129, an NDM-5-, CTX-M-14-, OXA-10- and MCR-1-co-producing Escherichia coli ST167 strain isolated from Japan.

J Glob Antimicrob Resist
  • Yoko Nukui
  • ,
  • Alafate Ayibieke
  • ,
  • Makoto Taniguchi
  • ,
  • Yoshibumi Aiso
  • ,
  • Yuka Shibuya
  • ,
  • Kazunari Sonobe
  • ,
  • Jun Nakajima
  • ,
  • Saki Kanehira
  • ,
  • Yoshiro Hadano
  • ,
  • Shuji Tohda
  • ,
  • Ryuji Koike
  • ,
  • Ryoichi Saito

18
開始ページ
148
終了ページ
150
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jgar.2019.07.001

The emergence and spread of carbapenemase-producing Enterobacteriaceae is a worldwide concern. This study reports the whole genome sequence of an NDM-5-, CTX-M-14-, OXA-10- and MCR-1-co-producing Escherichia coli sequence type 167 (ST167) multidrug-resistant clinical strain (EC129) isolated from a sputum sample of a hospitalised patient diagnosed with pneumonia.<br />
The genome of E. coli EC129 was subjected to next-generation sequencing and reads were assembled. The draft genome was annotated using DDBJ Read Annotation Pipeline DFAST server, followed by subsequent in silico analysis.<br />
and mcr-1 genes were detected along with other antimicrobial resistance genes conferring resistance to aminoglycosides, fluoroquinolones, sulfonamides, trimethoprim and tetracyclines. Antimicrobial susceptibility testing revealed that the isolate was resistant to all antimicrobial agents except colistin.<br />
To our knowledge, this study is the first to report anE. coli ST167 strain co-producing NDM-5, CTX-M-14, OXA-10 and MCR-1 isolated from a sputum sample of an individual with pneumonia in Japan, thus elucidating the molecular characteristics and resistance gene diversity of this strain.

リンク情報
DOI
https://doi.org/10.1016/j.jgar.2019.07.001
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31295582