2012年4月
Development and Validation of a Sensitive and Fast Solid-phase Spectrophotometric Procedure for Phenol Determination in Pharmaceuticals
ANALYTICAL SCIENCES
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- 巻
- 28
- 号
- 4
- 開始ページ
- 397
- 終了ページ
- 402
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.2116/analsci.28.397
- 出版者・発行元
- JAPAN SOC ANALYTICAL CHEMISTRY
A very sensitive, simple, and fast solid-phase spectrophotometric procedure for the determination of phenol using the p-nitrobenzenediazonium reagent (DAR reagent) was developed. This procedure is based on the simultaneous concentration of the orange product on a Dowex 1-X2 anion exchanger within 15 min, and a direct absorbance measurement of the sorbed species at both 530 nm (the absorption maximum of the phenol-DAR in the resin phase) and 700 nm (the range where only the resin absorbs light). Quality control and evaluation of the analytical parameters was carried out using a comprehensive prevalidation strategy. The linearity of the method was confirmed within an analyte working range from 0.01 to 0.10 mu mol (0.2 to 2.0 nmol mL(-1)). The precision ranged from +/- 1.17 to +/- 9.61%; the accuracy ranged from -17.50 to +17.81%. The evaluated limiting values were L-D = 0.0013 mu mol and L-Q = 0.0082 mu mol. The DAR-SPS method was successfully applied to the determination of phenol in a pharmaceutical sample of salicylic acid (98.0 - 100.0%) and vaccines (98.0 - 103.4%).
- リンク情報
- ID情報
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- DOI : 10.2116/analsci.28.397
- ISSN : 0910-6340
- eISSN : 1348-2246
- Web of Science ID : WOS:000303294700014