J-GLOBAL         Last updated: Aug 17, 2019 at 15:54
National Agriculture and Food Research Organization
Institute of Fruit Tree and Tea Science, NARO, Division of Citrus Research, Pest Management Unit
Job title
Principal Researcher
Research funding number

Research Areas


Published Papers

Differentiation of strains by the utilization of some carbohydrates and spatial distribution of citrus bacterial canker collected from a grove of lemon trees.
Kyushu Plant Protection Research   64 12-17   Nov 2018   [Refereed]
Iwanami, T. and Tomimura, K.
JARQ   51(3) 217-220   Jul 2017   [Refereed]
Ohshima, K., Nomiyama, R., Mitoma, S., Honda, Y., Yasaka, R. and Tomimura, K.
Infection, Genetics and Evolution   45 213-223   Nov 2016   [Refereed]
There is no attempt to evaluate evolutionary rates, timescales and diversities of viruses collected from mixedly infected hosts in nature. Plants of the genus Narcissus are a monocotyledon and are susceptible to several viruses. In this study, nar...
Inuma, T. and Tomimura, K.
Annual Report of The Kansai Plant Protection Society   58 107-108   Sep 2016   [Refereed]
Citrus mosaic virus (CiMV), a strain of Satsuma dwarf virus (SDV), was frequently detected on diseased citrus trees in Wakayama Prefecture using reverse transcription polymerase chain reaction analysis. Moreover, we surveyed 308 citrus trees from ...
Tomimura, K., Inoue, H. and Iwanami, T.
Applied Entomology and Zoology   49(2) 353-357   May 2014   [Refereed]
We developed nine simple sequence repeat (SSR) markers for the Japanese population of Diaphorina citri Kuwayama, which is known as the vector for citrus greening disease or huanglongbing, for use in population genetic studies. Analysis of 50 indiv...
Megumi Yoshida, Takashi Nakajima, Kenta Tomimura, Fumihiko Suzuki, Michiyoshi Arai, Atsushi Miyasaka
Plant Disease   96 845-851   Jan 2012   [Refereed]
Fungicide application to control Fusarium head blight (FHB) and accompanying mycotoxin contamination in wheat is generally performed at anthesis because wheat is most susceptible to FHB around this stage. In this study, we evaluated the effect of ...
Hiroshi Katoh, Siti Subandiyah, Kenta Tomimura, Mitsuru Okuda, Hong Ji Su, Toru Iwanami
Applied and Environmental Microbiology   77 1910-1917   Mar 2011
Four highly polymorphic simple sequence repeat (SSR) loci were selected and used to differentiate 84 Japanese isolates of "Candidatus Liberibacter asiaticus." The Nei's measure of genetic diversity values for these four SSRs ranged from 0.60 to 0....
Tomimura, K., Furuya, N., Miyata, S., Hamashima, A., Torigoe, H., Murayama, Y., Kawano, S., Okuda, M., Subandiyah, S., Su, H.-J., and Iwanami, T.
JARQ   44(2) 151-158   Apr 2010   [Refereed]
The Asian type “Candidatus Liberibacter asiaticus” (Las, citrus greening organism) is severely damaging citrus production in Asia including Japan. Our previous study suggested that the bacteriophage-type DNA polymerase region (DNA pol) would be u...
Noriko Furuya, Keiichiro Matsukura, Kenta Tomimura, Mitsuru Okuda, Shin ichi Miyata, Toru Iwanami
Journal of General Plant Pathology   76 122-131   Mar 2010   [Refereed]
In Japan and Southeast Asia, 'Candidatus Liberibacter asiaticus' (Las) is the dominant causal agent of citrus greening (huanglongbing) disease. Using PCR techniques, we determined the 11168-nucleotide sequence of the ψserA-trmU-tufB-secE-nusG-rplK...
Nakajima Takashi, Tomimura Kenta, Yoshida Megumi
Kyushu Pl. Prot Res.   55 25-30   Nov 2009   [Refereed]
赤かび病防除薬剤の再散布が必要な降雨条件を解明するため,人工降雨施設を用いたポット試験を行った。液剤( 2種)では各処理区とも降雨時間を 2時間,降雨強度を25mm/ 時とし,散布から降雨開始までの時間を 0-240分とした 5処理区を設けた。処理後,赤かび病菌を噴霧接種し,細霧ハウスに 1週間静置し,10日後に発病調査,収穫後にかび毒濃度を分析した。その結果,両剤ともに散布直後に降雨処理した場合のみ,発病度およびかび毒濃度について有意に防除効果が低下したが,散布30分以降の降雨であれば防...
Inoue, H., Ohnishi, J., Ito, T., Tomimura, K., Miyata, S., Iwanami, T., and Ashihara, W.
Annals of Applied Biology   155(2) 29-36   Oct 2009   [Refereed]
We carried out a quantitative detection of Candidatus Liberibacter asiaticus, the bacterium associated with the disease of huanglongbing, in the vector psyllid Diaphorina citri by using a TaqMan real-time PCR assay. The concentration of the bacter...
Tomimura, K., Miyata, S.-I., Furuya, N., Kubota, K., Okuda, M., Subandiyah, S., Hung, T.-H., Su, H.-J., and Iwanami, T.
Phytopathology   99(9) 1062-1069   Sep 2009   [Refereed]
The aim of this study was to investigate the genetic diversity and relationships among ‘Candidatus Liberibacter asiaticus’ isolates from different hosts and distinct geographical areas in Southeast Asia. Genetic diversity among ‘Ca. Liberibacter a...
Tomimura, K., Iwashita, K., Yamada, O., and Kobayashi, K.
Molecular Ecology Resources   9(2) 613-615   Mar 2009   [Refereed]
Using a bioinformatics approach, we developed 18 variable number of tandem repeat markers for Aspergillus oryzae for use in population genetic studies. Repeat sequences in the genome sequences of A. oryzae were identified by a tandem repeat findin...
Suzuki, F., Suga, H., Tomimura, K., Fuji, S., Arai, M., Koba, A., and Nakajima, T.
Molecular Ecology Resources   9(2) 588-590   Mar 2009   [Refereed]
We developed nine simple sequence repeat (SSR) markers useful for differentiating Japanese isolates of Magnaporthe grisea through a bioinformatic approach. Repeat sequences in the genome of M. grisea were identified by a Tandem Repeat Finding prog...
Karugia, GW., Suga, H., Gale, LR., Nakajima, T., Tomimura, K., and Hyakumachi, M.
Plant Disease   93(2) 170-174   Feb 2009   [Refereed]
The Fusarium graminearum species complex (Fg complex) that consists of at least 11 phylogenetically distinct species contains important Fusarium head blight (FHB) pathogens of wheat and barley worldwide. We obtained members of the Fg complex by sa...
Yoshida, M., Nakajima, T., Arai, M., Suzuki, F., and Tomimura, K.
Plant Disease   92(8) 1164-1170   Aug 2008   [Refereed]
Fungicide application is one measure available to reduce the risk of Fusarium head blight (FHB) and mycotoxin contamination in barley. The stage at or near anthesis, or at full head emergence, is generally thought to be optimal for fungicide appli...
Nakajima, T., Yoshida, M., and Tomimura, K.
Journal of General Plant Pathology   74(4) 289-295   Aug 2008   [Refereed]
Lodging is one possible risk factor that leads to increased cereal mycotoxin contamination, but few reports have been published on the subject. We examined the effects of lodging on the level of deoxynivalenol (DON) and nivalenol (NIV) contaminati...
Suga, H., Karugia, GW., Ward, T., Gale, LR., Tomimura, K., Nakajima, T., Miyasaka, A., Koizumi, S., Kageyama, K., and Hyakumachi, M.
Phytopathology   98(2) 159-166   Feb 2008   [Refereed]
Members of the Fusarium graminearum species complex are important cereal pathogens worldwide and belong to one of at least nine phylogenetically distinct species. We examined 298 strains of the F. graminearum species complex collected from wheat o...
Ohshima, K., Tomitaka, Y., Wood, JT., Minematsu, Y., Kajiyama, H., Tomimura, K., and Gibbs, A.
Journal of General Virology   88(1) 298-315   Jan 2007   [Refereed]
Potyviruses have variable single-stranded RNA genomes and many show clear evidence of recombination. This report studied the distribution of recombination sites in the genomes of 92 isolates of the potyvirus Turnip mosaic virus (TuMV); 42 came fro...
Ueda, S., Takeuchi, S., Okabayashi, M., Hanada, K., Tomimura, K., and Iwanami, T.
Journal of General Plant Pathology   71(4) 319-325   Aug 2005   [Refereed]
A new isolate of Tomato yellow leaf curl virus (TYLCV) has been identified from tomato plants in Kochi Prefecture in Japan and designated TYLCV-[Tosa]. The complete nucleotide sequence of the isolate was determined and found to consist of 2781 nt....
Tomimura, K., Spak, J., Katis, N., Jenner, CE., Walsh, JA., Gibbs, AJ., and Ohshima, K.
Virology   330(2) 408-423   Dec 2004   [Refereed]
The genetic structure of populations of Turnip mosaic virus in Eurasia was assessed by making host range and gene sequence comparisons of 142 isolates. Most isolates collected in West Eurasia infected Brassica plants whereas those from East Eurasi...
Jenner, CE., Wang, X., Tomimura, K., Ohshima, K., Ponz, F., and Walsh, JA.
Molecular Plant-Microbe Interactions   16(9) 777-784   Sep 2003   [Refereed]
Two isolates of the potyvirus Turnip mosaic virus (TuMV), UK 1 and CDN 1, differ both in their general symptoms on the susceptible propagation host Brassica juncea and in their ability to infect B. napus lines possessing a variety of dominant resi...
Tomimura, K., Gibbs, AJ., Jenner, CE., Walsh, JA., and Ohshima, K.
Molecular Ecology   12(8) 2099-2111   Aug 2003   [Refereed]
The genomes of a representative world-wide collection of 32 Turnip mosaic virus (TuMV) isolates were sequenced and these, together with six previously reported sequences, were analysed. At least one-fifth of the sequences were recombinant. In phyl...
Turnip Mosaic Virus determinants of virulence for Brassica napus resistance genes.
Jenner, CE., Sanchez, F., Tomimura, K., Ohshima, K., Ponz, F., and Walsh, JA
Plant Protection Science   38(1) 155-157   Sep 2002   [Refereed]
Dominant resistance genes identified in Brassica napus lines are effective against some, but not all, Turnip mosaic virus
(TuMV) isolates. An infectious clone of an isolate (UK 1) was used as the basis of chimeric virus constructions using
Jenner, CE., Tomimura, K., Ohshima, K., Hughes, SL., and Walsh, JA.
Virology   300(1) 50-59   Aug 2002   [Refereed]
The Brassica napus differential line 165 is resistant to infection by Turnip mosaic virus (TuMV) isolates belonging to pathotypes 1 and 3. Nucleotide sequences of resistance-breaking mutants of pathotype 1 (UK 1), pathotype 3 (CHN 12), and wild-ty...
Ohshima, K., Yamaguchi, Y., Hirota, R., Hamamoto, T., Tomimura, K., Tan, Z., Sano, T., Azuhata, F., Walsh, JA., Fletcher, J., Chen, J., Gera, A., and Gibbs, A.
Journal of General Virology   83(6) 1511-1521   Jun 2002   [Refereed]
Turnip mosaic virus (TuMV), a species of the genus Potyvirus, occurs worldwide. Seventy-six isolates of TuMV were collected from around the world, mostly from Brassica and Raphanus crops, but also from several non-brassica species. Host tests grou...


The current status of a measure against citrus greening disease and future prospect in Japan.
72(11) 694-698   Nov 2018   [Invited]
Molecular characterization of Satsuma dwarf virus strains collected in Japan
Citrus Research & Technology   31 78   Nov 2010

Research Grants & Projects

MAFF: Strategic project of MAFF
Project Year: Apr 2018 - Mar 2023    Investigator(s): SHIOTANI Hiroshi
NARO: Research program on development of innovative technology
Project Year: Apr 2019 - Mar 2022    Investigator(s): FUJIWARA Kazuki
Development of Detection and Identification Techniques of Pests in Research and Development for Global Warming Adaptation and Abnormal Weather Correspondence
Project Year: Jul 2015 - Mar 2020    Investigator(s): OHTO Yasuo
NARO: Research program on development of innovative technology
Project Year: Apr 2015 - Mar 2020    Investigator(s): KANEYOSHI Jyunko
MAFF: Research program on development of innovative technology
Project Year: Jun 2015 - Mar 2018    Investigator(s): FUJIKAWA Takashi
MAFF: Strategic project of MAFF
Project Year: Jun 2014 - Mar 2016    Investigator(s): IWANAMI Toru
MAFF: Research and development projects for application in promoting new policy of agriculture, MAFF
Project Year: Apr 2011 - Mar 2014    Investigator(s): MIYATA Shinichi
MAFF: Research and development projects for application in promoting new policy of agriculture, MAFF
Project Year: Apr 2010 - Mar 2013    Investigator(s): IWANAMI Toru
Evaluation of genetic diversity among Candidatus Liberibacter asiaticus isolates collected in Southeast Asia.
Ordinary Research
Project Year: 2007 - 2010