2020年11月12日
Simple transport and cryopreservation of cold-stored mouse embryos.
Experimental animals
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- 巻
- 69
- 号
- 4
- 開始ページ
- 423
- 終了ページ
- 429
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1538/expanim.20-0042
- 出版者・発行元
- Japanese Association for Laboratory Animal Science
The cold storage of two-cell embryos is a useful technique for transporting genetically engineered mice without the shipment of live animals. However, the developmental ability of cold-stored embryos decreases with prolonged storage periods. Therefore, the transported embryos must be readily transferred to recipient mice upon arrival. The cryopreservation of cold-transported embryos may improve the flexibility of the schedule of embryo transfer. In this paper, we examined the viability and developmental ability of vitrified-warmed mouse embryos at the two-cell stage after cold storage in refrigerated temperatures for 0, 24, 48, 72, or 96 h. The viability of vitrified-warmed embryos after cold storage was comparable to vitrified-warmed embryos without cold storage. Vitrified-warmed embryos after cold storage also developed normally to pups by embryo transfer. In addition, live pups were obtained from vitrified-warmed embryos after cold-transportation from Asahikawa Medical University. In summary, cold-stored embryos can be used for the transportation and archive of genetically engineered mice.
- リンク情報
- ID情報
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- DOI : 10.1538/expanim.20-0042
- ISSN : 1341-1357
- eISSN : 1881-7122
- PubMed ID : 32554941
- PubMed Central 記事ID : PMC7677088