The eggshells of 56 chelonians were examined by electron microscopy and X-ray diffractometry. They were classified into six types in terms of the matrix structure of their calcareous layer; type I was composed of a thin calcareous layer with miner...
Journal of Reproduction and Development 57(2) 262-266 Apr 2011
The purposes of the present study were to establish a noninvasive monitoring assay of fecal progestagen measurement to detect pregnancy and to identify the components of fecal progestagens in early, middle and late pregnancy in cheetahs. Feces wer...
The structure and mineral composition of eggshell cuticles were studied in 5 species of birds. The approximate thickness of the cuticle layer at the top of shell columns was about 1 μm in the Red Junglefowl (Gallus gallus), about 130 μm in the Whi...
I. Adachi, S. Kusuda, E. Nagao, Y. Taira, M. Asano, T. Tsubota, O. Doi
Theriogenology 74(8) 1499-1503 Nov 2010
Although the Tsushima leopard cat (Prionailurus bengalensis euptilurus) is one of the most endangered mammals in Japan, its reproductive physiology and endocrinology have been not elucidated. The objective was to establish the non-invasive monitor...
Satoshi Kusuda, Yuka Kakizoe, Koji Kanda, Tomoko Sengoku, Yohei Fukumoto, Itsuki Adachi, Yoko Watanabe, Osamu Doi
Zoo Biology 30(3) 285-295 Jul 2010
This study aimed to validate the measurements of body temperature and fecal progesterone concentrations as minimally invasive techniques for assessing ovarian cycle in a single sexually mature female killer whale. Rectal temperature data, fecal an...
Detection of estrous cycle and pregnancy based on sexual behaviors and fecal progesterone profile in the Arabian oryx, Oryx leucoryx
Journal of Reproduction and Development 53(6) 1283-1289 Dec 2007
The progesterone (P4) profiles and macroscopic vulvar changes of female Malayan tapirs were investigated in order to understand their fundamental reproductive physiology and to search for visual indicators of estrus. Blood was collected once or tw...
Satoshi Kusuda, Koki Morikaku, Ken Ichi Kawada, Kenji Ishiwada, Kenji Ishiwada, Osamu Doi
Journal of Reproduction and Development 53(1) 143-150 Feb 2007
The aim of the present study was to establish a simple method to monitor ovarian activity and non-invasively diagnose pregnancy in okapi (Okapia johnstoni). The feces of a female okapi were collected daily or every 3 days for 28 months. Steroids i...
Journal of Veterinary Medical Science 68(10) 1093-1095 Oct 2006
The serum and fecal testosterone (T) concentrations and testicular sizes of two male bharals (Pseudois nayaur) were determined for approximately one year. The profiles of the fecal T concentrations showed a similar tendency as the profiles of seru...
Journal of Veterinary Medical Science 68(8) 847-851 Aug 2006
The purpose of present study was to determine annual changes in serum progesterone (P4) concentrations and to clarify basic reproductive characteristics, such as breeding season, estrous cycle, and puberty in female bharals (Pseudois nayaur). Bloo...
Studies on the reproductive endocrinology of koalas have been performed mainly by using blood samples; however, in practice it is difficult to collect blood periodically because koalas are easily stressed. The purposes of the present study were to...
Journal of Veterinary Medical Science 69(5) 569-571
Feces were collected from two female and one male Siberian tigers, Panthera tigris altaica. Steroid hormones were extracted from lyophilized feces and quantified by enzyme immunoassay. The fecal contents of estradiol-17 β(E2) and testosterone in t...
The objective of this study was to clarify the current status of endangered Kiso horse, population statistics and biological traits, in order to take a step for the conservation by scientific approach. We surveyed 125 Kiso horses (86.2% of the who...
The objective of this study was to clarify the current status of endangered Kiso horse, population statistics and biological traits, in order to take a step for the conservation by scientific approach. We surveyed 125 Kiso horses (86.2% of the who...
Journal of Reproduction and Development 53(5) 1099-1105
The aim of the present study was to investigate whether changes in body temperature are related to the ovarian cycle in elephants. Rectal, tongue or fecal temperature was measured for 2 Asian and 5 African elephants using an electric thermometer. ...
This study aimed at demonstrating the profiles of circulating gonadal steroid hormones during the estrous cycle and pregnancy in a southern tamandua (Tamandua tetradactyla). Additionally, this study clarified the relationship between vulvar bleedi...
Information about breeding and the reproductive biology of mouse deer is limited in the wild and captivity No reports on reproductive endocrinology are available The objective of the present study was to observe the reproductive biology based on b...
Satoshi Kusuda, Hisashi Hashikawa, Masato Takeda, Hideki Ito, Atsushi Goto, Jun Oguchi, Osamu Doi
Journal of Reproduction and Development 59 308-313
The purposes of the present study were to clarify age- and season- related androgen patterns, and to compare the reproductive physiology between Japanese captive koala populations and Australian populations. To measure fecal androgens, feces were ...
Reproductive physiology and analysis method for reproductive status survey were studied for the conservation of endangered carnivores such as bear and felid species. As a resultnon-invasiveendocrine monitoring method using feces were established f...
Development of non-invasive endocrine monitoring techniques for endangered mammals
