論文

国際誌
2021年10月22日

Crosstalk between Cancer Cells and Fibroblasts for the Production of Monocyte Chemoattractant Protein-1 in the Murine 4T1 Breast Cancer.

Current issues in molecular biology
  • Mayu Imamura
  • ,
  • Tiantian Li
  • ,
  • Chunning Li
  • ,
  • Masayoshi Fujisawa
  • ,
  • Naofumi Mukaida
  • ,
  • Akihiro Matsukawa
  • ,
  • Teizo Yoshimura

43
3
開始ページ
1726
終了ページ
1740
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/cimb43030122

The chemokine monocyte chemoattractant protein-1 (MCP-1/CCL2) is shown to promote the progression of breast cancer. We previously identified cancer cell-derived granulocyte-macrophage colony-stimulating factor (GM-CSF) as a potential regulator of MCP-1 production in the murine 4T1 breast cancer, but it played a minimum role in overall MCP-1 production. Here, we evaluated the crosstalk between 4T1 cells and fibroblasts. When fibroblasts were co-cultured with 4T1 cells or stimulated with the culture supernatants of 4T1 cells (4T1-sup), MCP-1 production by fibroblasts markedly increased. 4T1 cells expressed mRNA for platelet-derived growth factor (PDGF)-a, b and c, and the PDGF receptor inhibitor crenolanib almost completely inhibited 4T1-sup-induced MCP-1 production by fibroblasts. However, PDGF receptor antagonists failed to reduce MCP-1 production in tumor-bearing mice. Histologically, 4T1 tumors contained a small number of αSMA-positive fibroblasts, and Mcp-1 mRNA was mainly associated with macrophages, especially those surrounding necrotic lesions on day 14, by in situ hybridization. Thus, although cancer cells have the capacity to crosstalk with fibroblasts via PDGFs, this crosstalk does not play a major role in MCP-1 production or cancer progression in this model. Unraveling complex crosstalk between cancer cells and stromal cells will help us identify new targets to help treat breast cancer patients.

リンク情報
DOI
https://doi.org/10.3390/cimb43030122
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34698088
ID情報
  • DOI : 10.3390/cimb43030122
  • PubMed ID : 34698088

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