2000年3月
Lazaroid reduces production of IL-8 and IL-1 receptor antagonist in ischemic spinal cord injury
ANNALS OF THORACIC SURGERY
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- 巻
- 69
- 号
- 3
- 開始ページ
- 792
- 終了ページ
- 798
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/S0003-4975(99)01413-7
- 出版者・発行元
- ELSEVIER SCIENCE INC
Background. 21-aminosteroids (lazaroids) have demonstrated the protective effect against cerebral ischemic injury through the inhibition of lipid peroxidation. We examined whether lazaroids affected the production of proinflammatory and antiinflammatory cytokines in ischemic spinal cord injury model.
Materials. Anesthetized New Zealand white rabbits underwent a 20-minute infrarenal aortic cross-clamping (AXC) with pretreatment of either an intravenous 3 mg/kg lazaroid U74389G (group L; n = 10) or the same volume saline (group P; n = 10), Sham operation group (group S; n = 6) underwent only exposure of the aorta. Plasma concentrations of interleukin (IL)-8, -1 beta, -1 receptor antagonist (IL-1ra) and tumor necrosis factor (TNF)-alpha were measured at four time points. Functional assessment with Tarlov score at 24 and 48 hours after pretreatment, pathologic assessment of the spinal cord, and measurements of cytokine levels in the spinal cord were performed.
Results, The maximum elevation of plasma IL-8 and -1ra levels occurred at 1 hour after declamping in four measurement points, Plasma IL-8 and -1ra levels in group L were significantly lower than those in group P (*p < 0.05), Plasma TNF alpha peaked at 5 minutes after declamping, but decreased afterwards. Plasma TNF alpha levels were not different among three groups, Spinal IL-8 levels in group L (0.98 +/- 0.34 ng/g tissue) were lower than those in group P (7.26 +/- 2.26 ng/g tissue) (*p < 0.05), Spinal IL-1ra and TNF alpha were not significantly different Tarlov scare and pathologic assessment were better in group L,
Conclusions. Lazaroid U-74389G reduced the production of systemic IL-8 and -1ra and spinal IL-8 when AXC. caused spinal cord injury. These results indicate that lazaroids may attenuate ischemic endothelial cell injury or activation of leukocytes,
(C) 2000 by The Society of Thoracic Surgeons.
Materials. Anesthetized New Zealand white rabbits underwent a 20-minute infrarenal aortic cross-clamping (AXC) with pretreatment of either an intravenous 3 mg/kg lazaroid U74389G (group L; n = 10) or the same volume saline (group P; n = 10), Sham operation group (group S; n = 6) underwent only exposure of the aorta. Plasma concentrations of interleukin (IL)-8, -1 beta, -1 receptor antagonist (IL-1ra) and tumor necrosis factor (TNF)-alpha were measured at four time points. Functional assessment with Tarlov score at 24 and 48 hours after pretreatment, pathologic assessment of the spinal cord, and measurements of cytokine levels in the spinal cord were performed.
Results, The maximum elevation of plasma IL-8 and -1ra levels occurred at 1 hour after declamping in four measurement points, Plasma IL-8 and -1ra levels in group L were significantly lower than those in group P (*p < 0.05), Plasma TNF alpha peaked at 5 minutes after declamping, but decreased afterwards. Plasma TNF alpha levels were not different among three groups, Spinal IL-8 levels in group L (0.98 +/- 0.34 ng/g tissue) were lower than those in group P (7.26 +/- 2.26 ng/g tissue) (*p < 0.05), Spinal IL-1ra and TNF alpha were not significantly different Tarlov scare and pathologic assessment were better in group L,
Conclusions. Lazaroid U-74389G reduced the production of systemic IL-8 and -1ra and spinal IL-8 when AXC. caused spinal cord injury. These results indicate that lazaroids may attenuate ischemic endothelial cell injury or activation of leukocytes,
(C) 2000 by The Society of Thoracic Surgeons.
- リンク情報
- ID情報
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- DOI : 10.1016/S0003-4975(99)01413-7
- ISSN : 0003-4975
- Web of Science ID : WOS:000086022000033