Dec, 2000
Neutrophil infiltration as a crucial step for monocyte chemoattractant protein (MCP)-1 to attract monocytes in lipopolysaccharide-induced arthritis in rabbits
INFLAMMATION RESEARCH
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- Volume
- 49
- Number
- 12
- First page
- 673
- Last page
- 678
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1007/s000110050645
- Publisher
- BIRKHAUSER VERLAG AG
Objective and Design: To evaluate the mechanism whereby monocyte chemoattractant protein (MCP)-1 attracts monocytes in vivo.
Subjects: New Zealand white rabbits (175 rabbits) were used.
Treatment: LPS, MCP-1 or IL-8 was injected into knee joints. Antibodies against various cytokines or IL-1 receptor antagonist were injected to neutralize cytokine activities.
Methods: The numbers of leukocyte populations, levels of cytokines in joints were estimated.
Results: Partial inhibition of neutrophil influx with anti-IL-8 IgG (10 mug) suppressed LPS-induced macrophage influx by 43 +/- 8.5% (p<0.05) without affecting the MCP-I level. Intraarticular injection of MCP-1 (1-30 <mu>g) induced macrophage influx. The event was accompanied by a small number of neutrophils in an early phase. Go-injection of IL-8 (1.0 mug) enhanced the MCP-1-induced macrophage infiltration (p<0.01). In neutrophil-depleted rabbits, LPS failed to induce macrophage influx even though the MCP-1 level was maintained, and macrophage influx following exogenously administered MCP-1 was also dramatically inhibited.
Conclusions: Early events associated with neutrophil infiltration appear to be important for MCP-1 to induce a later macrophage influx in LPS-arthritis.
Subjects: New Zealand white rabbits (175 rabbits) were used.
Treatment: LPS, MCP-1 or IL-8 was injected into knee joints. Antibodies against various cytokines or IL-1 receptor antagonist were injected to neutralize cytokine activities.
Methods: The numbers of leukocyte populations, levels of cytokines in joints were estimated.
Results: Partial inhibition of neutrophil influx with anti-IL-8 IgG (10 mug) suppressed LPS-induced macrophage influx by 43 +/- 8.5% (p<0.05) without affecting the MCP-I level. Intraarticular injection of MCP-1 (1-30 <mu>g) induced macrophage influx. The event was accompanied by a small number of neutrophils in an early phase. Go-injection of IL-8 (1.0 mug) enhanced the MCP-1-induced macrophage infiltration (p<0.01). In neutrophil-depleted rabbits, LPS failed to induce macrophage influx even though the MCP-1 level was maintained, and macrophage influx following exogenously administered MCP-1 was also dramatically inhibited.
Conclusions: Early events associated with neutrophil infiltration appear to be important for MCP-1 to induce a later macrophage influx in LPS-arthritis.
- Link information
- ID information
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- DOI : 10.1007/s000110050645
- ISSN : 1023-3830
- Web of Science ID : WOS:000166814100004