論文

査読有り
2014年1月

Biofilm Formation under Different Temperature Conditions by a Single Genotype of Persistent Listeria monocytogenes Strains

JOURNAL OF FOOD PROTECTION
  • Yoshitsugu Ochiai
  • ,
  • Fumiya Yamada
  • ,
  • Mariko Mochizuki
  • ,
  • Takashi Takano
  • ,
  • Ryo Hondo
  • ,
  • Fukiko Ueda

77
1
開始ページ
133
終了ページ
140
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.4315/0362-028X.JFP-13-074
出版者・発行元
INT ASSOC FOOD PROTECTION

Some Listeria monocyto genes strains, termed persistent strains, originate from the same processing plant and have the ability to survive and grow over extended periods of time at contamination sources. In order to evaluate biofilm formation by such persistent strains, we isolated the pathogen from chicken samples collected from the same retail shop in repeated visits over 6 months. Strains that were of serotype 1/2b and were assigned to the same genotype by multi-virulence-locus sequence typing analysis were isolated on repeated occasions from December 1997 to June 1998 and thus were defined as persistent strains. In the present study, biofilm formation by the persistent strains was evaluated using microplates at 30 and 37 degrees C. The biofilm-forming capability was measured after cells attaching to the microplate well were stained with crystal violet. Comparison of biofilm formation at 30 degrees C among the persistent strains showed that a significantly higher amount of the stain was obtained from the persistent strains isolated from December to March than from those isolated from April to June. However, no significant difference in biofilm formation at 30 degrees C was observed between persistent and nonpersistent groups of L. monocyto genes strains. In contrast, biofilm formation at 37 degrees C was consistent among the persistent strains, and they produced significantly more biofilm at 37 degrees C than did the nonpersistent strains. The persistent strains were also found to change their biofilm-forming ability in a temperature-dependent manner, which may suggest that the persistent strains alter their biofilm formation in response to changing environmental factors.

リンク情報
DOI
https://doi.org/10.4315/0362-028X.JFP-13-074
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24406011
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000329593300019&DestApp=WOS_CPL
ID情報
  • DOI : 10.4315/0362-028X.JFP-13-074
  • ISSN : 0362-028X
  • eISSN : 1944-9097
  • PubMed ID : 24406011
  • Web of Science ID : WOS:000329593300019

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