2020年3月
Structural effect of amine N-oxides on the facilitation of alpha-glucosidase-catalyzed hydrolysis reactions
BIOPROCESS AND BIOSYSTEMS ENGINEERING
- ,
- ,
- ,
- 巻
- 43
- 号
- 3
- 開始ページ
- 541
- 終了ページ
- 548
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s00449-019-02248-w
- 出版者・発行元
- SPRINGER
Activation and stabilization of enzymes is an important issue in their industrial application. We recently reported that synthetic betaines, derived from cellular metabolites, structure-dependently increased the activity and stability of various enzymes including hydrolases, oxidases, and synthetases simply by mixing them into the reaction buffer. In this report, we focus on amine N-oxides, which are similarly important metabolites in cells with a highly polarized N-oxide bond, and investigate their enzyme stabilization and activation behavior. It was revealed that synthetic amine N-oxides structure-dependently activate alpha-glucosidase-catalyzed hydrolysis reactions similarly to betaines. The subsequent comparison of the kinetic parameters, the optimal concentration range for activation, and the maximal activity, suggested that amine N-oxides facilitate hydrolysis reactions via the same mechanism as betaines, because no differences were confirmed. However, the enzyme stabilization effect of amine N-oxides was slightly superior to that of betaines and the temporal stability of the enzyme in aqueous solutions was higher in the low amine N-oxide concentration range. The rheological properties, CD spectra, and dynamic fluorescence quenching experiments suggested that the suppression of unfavorable conformational perturbation was related to the difference in the hydration environments provided by the surrounding water molecules. Thus, we clarified that amine N-oxides facilitate enzyme reactions as a result of their similarity to betaines and provide a superior stabilizing effect for enzymes. Amine N-oxides show potential for application in enzyme storage and long-term reactions.
- リンク情報
- ID情報
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- DOI : 10.1007/s00449-019-02248-w
- ISSN : 1615-7591
- eISSN : 1615-7605
- PubMed ID : 31741084
- Web of Science ID : WOS:000511789600017