2016年1月
Patterned Co-culture of Live Cells on a Microchip by Photocrosslinking with Benzophenone
ANALYTICAL SCIENCES
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- 巻
- 32
- 号
- 1
- 開始ページ
- 113
- 終了ページ
- 116
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.2116/analsci.32.113
- 出版者・発行元
- JAPAN SOC ANALYTICAL CHEMISTRY
The patterned coculture of different types of living cells in a microfluidic device is crucial for the analysis of cellular interactions and cell-cell communication. In the present study, cell patterning was achieved by photocrosslinking benzophenone derivatives in a microfluidic channel. Optimization of UV irradiation conditions enabled successful fixation of live cells. In addition, patterning and co-culture of non-adherent K562 cells and adherent RF-6A cells was achieved by successive rounds of patterning. The present approach is expected to be useful for the development of in vitro methods for studying cell signaling.
- リンク情報
- ID情報
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- DOI : 10.2116/analsci.32.113
- ISSN : 0910-6340
- eISSN : 1348-2246
- Web of Science ID : WOS:000368963300019