Papers

Peer-reviewed
Jul, 2012

A palmtop-sized microfluidic cell culture system driven by a miniaturized infusion pump

ELECTROPHORESIS
  • Naoki Sasaki
  • ,
  • Mika Shinjo
  • ,
  • Satoshi Hirakawa
  • ,
  • Masahiro Nishinaka
  • ,
  • Yo Tanaka
  • ,
  • Kazuma Mawatari
  • ,
  • Takehiko Kitamori
  • ,
  • Kae Sato

Volume
33
Number
12
First page
1729
Last page
1735
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1002/elps.201100691
Publisher
WILEY-BLACKWELL

A palmtop-sized microfluidic cell culture system is presented. The system consists of a microfluidic device and a miniaturized infusion pump that possesses a reservoir of culture medium, an electrical control circuit, and an internal battery. The footprint of the system was downsized to 87 x 57 mm, which is, to the best of our knowledge, the smallest integrated cell culture system. Immortalized human microvascular endothelial cells (HMEC-1) and human umbilical vein endothelial cells (HUVEC) were cultured in the system. HMEC-1 in the system proliferated at the same speed as cells in a microchannel perfused by a syringe pump and cells in a culture flask. HUVEC in the system oriented along the direction of the fluid flow. Claudin-5, a tight junction protein, was localized along the peripheries of the HUVEC. We expect that the present system is applicable to various cell types as a stand-alone and easy-to-use system for microfluidic bioanalysis.

Link information
DOI
https://doi.org/10.1002/elps.201100691
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22740461
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000305792500008&DestApp=WOS_CPL
ID information
  • DOI : 10.1002/elps.201100691
  • ISSN : 0173-0835
  • Pubmed ID : 22740461
  • Web of Science ID : WOS:000305792500008

Export
BibTeX RIS