2012年7月
A palmtop-sized microfluidic cell culture system driven by a miniaturized infusion pump
ELECTROPHORESIS
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 33
- 号
- 12
- 開始ページ
- 1729
- 終了ページ
- 1735
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/elps.201100691
- 出版者・発行元
- WILEY-BLACKWELL
A palmtop-sized microfluidic cell culture system is presented. The system consists of a microfluidic device and a miniaturized infusion pump that possesses a reservoir of culture medium, an electrical control circuit, and an internal battery. The footprint of the system was downsized to 87 x 57 mm, which is, to the best of our knowledge, the smallest integrated cell culture system. Immortalized human microvascular endothelial cells (HMEC-1) and human umbilical vein endothelial cells (HUVEC) were cultured in the system. HMEC-1 in the system proliferated at the same speed as cells in a microchannel perfused by a syringe pump and cells in a culture flask. HUVEC in the system oriented along the direction of the fluid flow. Claudin-5, a tight junction protein, was localized along the peripheries of the HUVEC. We expect that the present system is applicable to various cell types as a stand-alone and easy-to-use system for microfluidic bioanalysis.
- リンク情報
- ID情報
-
- DOI : 10.1002/elps.201100691
- ISSN : 0173-0835
- PubMed ID : 22740461
- Web of Science ID : WOS:000305792500008