Papers

Peer-reviewed
Jun, 2012

Photochemical Immobilization of Cells onto a Glass Substrate for in situ DNA Analysis

ANALYTICAL SCIENCES
  • Naoki Sasaki
  • ,
  • Anri Isu
  • ,
  • Reina Ishii
  • ,
  • Kae Sato

Volume
28
Number
6
First page
537
Last page
539
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.2116/analsci.28.537
Publisher
JAPAN SOC ANALYTICAL CHEMISTRY

A simple and robust method to immobilize cells onto a glass substrate is presented. The method employs a photochemical reaction of benzophenone, which is modified on the substrate using a standard silane coupling agent, with cells. Cells were immobilized to an area irradiated with UV light from a standard light source under an inverted microscope. The dependence of immobilization on the light power intensity and irradiation time was investigated. In situ DNA analysis within the immobilized cells was demonstrated using target-primed rolling circle amplification and fluorescent detection.

Link information
DOI
https://doi.org/10.2116/analsci.28.537
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000305872500001&DestApp=WOS_CPL
ID information
  • DOI : 10.2116/analsci.28.537
  • ISSN : 0910-6340
  • Web of Science ID : WOS:000305872500001

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