論文

査読有り
2012年2月

Rare sugar D-allose strongly induces thioredoxin-interacting protein and inhibits osteoclast differentiation in Raw264 cells

NUTRITION RESEARCH
  • Kana Yamada
  • ,
  • Chisato Noguchi
  • ,
  • Kazuyo Kamitori
  • ,
  • Youyi Dong
  • ,
  • Yuko Hirata
  • ,
  • Mohammad A. Hossain
  • ,
  • Ikuko Tsukamoto
  • ,
  • Masaaki Tokuda
  • ,
  • Fuminori Yamaguchi

32
2
開始ページ
116
終了ページ
123
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.nutres.2011.12.010
出版者・発行元
PERGAMON-ELSEVIER SCIENCE LTD

Oxidative stress modulates the osteoclast differentiation via redox systems, and thiorcdoxin 1 (Trx) promotes the osteoclast formation by regulating the activity of transcription factors. The function of Trx is known to be regulated by its binding partner, thioredoxin-interacting protein (TXNIP). We previously reported that the expression of TXNIP gene is strongly induced by a rare sugar D-allose. In this study, we tested the hypothesis that D-allose could inhibit the osteoclast differentiation by regulating the Trx function. We used a murine Raw264 cell line that differentiates to the osteoclast by the receptor activator of nuclear factor-kappa B ligand (RANKL) treatment. The effect of sugars was evaluated by tartrate-resistant acid phosphatase staining. The expression and localization of TXNIP and Trx protein were examined by Western blotting and immunohistochemisty. The activity of the nuclear factor-kappa B, nuclear factor of activated T cells, and activator protein 1 transcription factors was measured by the luciferase reporter assay. The addition of D-allose (25 mmol/L) inhibited the osteoclast differentiation down to 9.53% +/- 1.27% of a receptor activator of nuclear factor-kappa B ligand only treatment. During the osteoclast differentiation, a significant increase of TNXIP was observed by D-allose treatment. The immunohistochcmical analysis showed that both Trx and TXNIP existed in the nucleus in preosteoclasts and osteoclasts. Overexpression of TXNIP by plasmid transfection also inhibited the osteoclast formation, indicating the functional importance of TXNIP for the osteoelast differentiation. Transcriptional activity of the activator protein 1, nuclear factor-kappa B, and nuclear factor of activated T cells, known to be modulated by Trx, were inhibited by D-allose. In conclusion, our data indicate that D-allose is a strong inhibitor of the osteoclast differentiation, and this effect could be caused by TXNIP induction and a resulting inhibition of the Trx function. (C) 2012 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.nutres.2011.12.010
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000301008400007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.nutres.2011.12.010
  • ISSN : 0271-5317
  • Web of Science ID : WOS:000301008400007

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