論文

2013年4月

Degradation of initiator tRNA(Met) by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells

NUCLEIC ACIDS RESEARCH
  • Kazunori Watanabe
  • ,
  • Ryu Miyagawa
  • ,
  • Chie Tomikawa
  • ,
  • Rie Mizuno
  • ,
  • Akihisa Takahashi
  • ,
  • Hiroyuki Hori
  • ,
  • Kenichi Ijiri

41
8
開始ページ
4671
終了ページ
4685
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/nar/gkt153
出版者・発行元
OXFORD UNIV PRESS

Stress response mechanisms that modulate the dynamics of tRNA degradation and accumulation from the cytoplasm to the nucleus have been studied in yeast, the rat hepatoma and human cells. In the current study, we investigated tRNA degradation and accumulation in HeLa cells under various forms of stress. We found that initiator tRNA(Met) (tRNA(iMet)) was specifically degraded under heat stress. Two exonucleases, Xrn1 and Xrn2, are involved in the degradation of tRNA(iMet) in the cytoplasm and the nucleus, respectively. In addition to degradation, we observed accumulation of tRNA(iMet) in the nucleus. We also found that the mammalian target of rapamycin (mTOR), which regulates tRNA trafficking in yeast, is partially phosphorylated at Ser2448 in the presence of rapamycin and/or during heat stress. Our results suggest phosphorylation of mTOR may correlate with accumulation of tRNA(iMet) in heat-treated HeLa cells.

Web of Science ® 被引用回数 : 17

リンク情報
DOI
https://doi.org/10.1093/nar/gkt153
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000318569700034&DestApp=WOS_CPL

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