論文

査読有り
2013年10月16日

Near-infrared light-directed RNAi using a photosensitive carrier molecule

Bioconjugate Chemistry
  • Yuka Matsushita-Ishiodori
  • ,
  • Mika Morinaga
  • ,
  • Kazunori Watanabe
  • ,
  • Takashi Ohtsuki

24
10
開始ページ
1669
終了ページ
1673
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/bc4001195

Controlled activation of small RNAs, such as small interfering RNA, in cells is very useful for various biological applications. Light is an effective inducer of controlled activation
in particular, near-infrared light is favorable because it can penetrate deeper into tissues than UV or visible light. In this study, near-infrared light control of RNA interference (RNAi) was demonstrated in mammalian cells using a photosensitive RNA carrier molecule, consisting of an RNA carrier protein and a fluorochrome. The photosensitive carrier molecule was identified from six candidates, each with a different fluorochrome. Using this carrier molecule, cytosolic RNA delivery and RNAi can be triggered by near-infrared light. Cytotoxicity was not observed after photoinduction of RNAi. © 2013 American Chemical Society.

リンク情報
DOI
https://doi.org/10.1021/bc4001195
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23998791

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