論文

2016年8月

Phototriggered protein syntheses by using (7-diethylaminocoumarin-4-yl)methoxycarbonyl-caged aminoacyl tRNAs

NATURE COMMUNICATIONS
  • Takashi Ohtsuki
  • ,
  • Shigeto Kanzaki
  • ,
  • Sae Nishimura
  • ,
  • Yoshio Kunihiro
  • ,
  • Masahiko Sisido
  • ,
  • Kazunori Watanabe

7
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/ncomms12501
出版者・発行元
NATURE PUBLISHING GROUP

The possibility of spatiotemporally photocontrolling translation holds considerable promise for studies on the biological roles of local translation in cells and tissues. Here we report caged aminoacyl-tRNAs (aa-tRNAs) synthesized using a (7-diethylaminocoumarin-4-yl) methoxycarbonyl (DEACM)-cage compound. DEACM-caged aa-tRNA does not spontaneously deacylate for at least 4 h in neutral aqueous solution, and does not bind to the elongation factor Tu. On irradiation at similar to 405 nm at 125mWcm(-2), DEACM-aa-tRNA is converted into active aa-tRNA with a half-life of 19 s. Notably, this rapid uncaging induced by visible light does not impair the translation system. Translation is photoinduced when DEACM-aa-tRNA carrying a CCCG or a CUA anticodon is uncaged in the presence of mRNAs harbouring a CGGG four-base codon or a UAG amber codon, respectively. Protein synthesis is phototriggered in several model systems, including an in vitro translation system, an agarose gel, in liposomes and in mammalian cells.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1038/ncomms12501
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000381771700001&DestApp=WOS_CPL

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