論文

2017年5月

Detection of small, highly structured RNAs using molecular beacons

ANALYTICAL METHODS
  • J. Li
  • ,
  • C. Xu
  • ,
  • N. Shimada
  • ,
  • Y. Miyoshi
  • ,
  • K. Watanabe
  • ,
  • W. Cong
  • ,
  • T. Ohtsuki

9
20
開始ページ
2971
終了ページ
2976
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1039/c7ay00341b
出版者・発行元
ROYAL SOC CHEMISTRY

The use of molecular beacons is a versatile method to detect RNAs. Typically, a single-stranded region of RNA is selected as a target sequence for molecular beacons. Therefore, the detection of highly structured short RNAs, such as tRNAs, seems to be difficult. In this study, as an example of highly structured target RNA, we used human tRNA(Lys3), which is known to have functions in protein synthesis and the reverse transcription of human immunodeficiency virus (HIV)-1. No long single-stranded region more than 8 nt is present in this tRNA, which is much shorter than the standard target length of molecular beacons (similar to 20 nt). This study showed that sensitive detection of highly structured RNAs using molecular beacons was much more difficult than that of unstructured or less structured RNAs. However, efficient detection of the tRNA(Lys3) was achieved by selecting the best target region, i.e., the region around the D arm, probably due to the ease of unfolding of this arm. Accordingly, our findings suggested that molecular beacons may have applications in the detection of highly structured RNAs related to various biological functions and diseases.

Web of Science ® 被引用回数 : 2

リンク情報
DOI
https://doi.org/10.1039/c7ay00341b
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000402044500006&DestApp=WOS_CPL

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