論文

査読有り
2016年

Enhanced cellular uptake of lactosomes using cell-penetrating peptides

SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
  • Akiya Akahoshi
  • ,
  • Eiji Matsuura
  • ,
  • Eiichi Ozeki
  • ,
  • Hayato Matsui
  • ,
  • Kazunori Watanabe
  • ,
  • Takashi Ohtsuki

17
1
開始ページ
245
終了ページ
252
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/14686996.2016.1178056
出版者・発行元
TAYLOR & FRANCIS LTD

Polymeric micelles that are composed of synthetic polymers are generally size controllable and can be easily modified for various applications. Lactosomes (A(3)B-type) are biodegradable polymeric micelles composed of an amphipathic polymer, including three poly(sarcosine) blocks and a poly(L-lactic acid) block. Lactosomes accumulate in tumors in vivo through the enhanced permeability and retention (EPR) effect, even on frequently administering them. However, lactosomes cannot be efficiently internalized by cells. To improve cellular uptake of lactosomes, cell-penetrating peptide (CPP)-modified lactosomes were prepared. Seven CPPs (including EB1 and Pep1) were used, and most of them improved the cellular uptake efficiency of lactosomes. In particular, EB1- and Pep1-modified lactosomes were efficiently internalized by cells. In addition, by using CPP-modified and photosensitizer-loaded lactosomes, we demonstrated the photoinduced killing of mammalian cells, including human cancer cells. Accumulation of the EB1-modified lactosomes in NCI-N87 tumors was shown by in vivo imaging. Thus, this study demonstrated that the CPP-modified lactosome is a promising drug carrier.
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Web of Science ® 被引用回数 : 2

リンク情報
DOI
https://doi.org/10.1080/14686996.2016.1178056
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000379494500001&DestApp=WOS_CPL

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