MISC

2011年2月

Stage-specific roles of fibulin-5 during oxidative stress-induced renal carcinogenesis in rats

FREE RADICAL RESEARCH
  • Hiroki Ohara
  • ,
  • Shinya Akatsuka
  • ,
  • Hirotaka Nagai
  • ,
  • Yu-Ting Liu
  • ,
  • Li Jiang
  • ,
  • Yasumasa Okazaki
  • ,
  • Yoriko Yamashita
  • ,
  • Tomoyuki Nakamura
  • ,
  • Shinya Toyokuni

45
2
開始ページ
211
終了ページ
220
記述言語
英語
掲載種別
DOI
10.3109/10715762.2010.523702
出版者・発行元
INFORMA HEALTHCARE

By using a rat model of renal cell carcinoma (RCC) induced by ferric nitrilotriacetate (Fe-NTA), this study performed genome-wide analysis to identify target genes during carcinogenesis. It screened for genes with decreased expression in RCCs, with simultaneous loss of heterozygosity, eventually to focus on the fibulin-5 (fbln5) gene. Oxidative damage via Fe-NTA markedly increased Fbln5 in the proximal tubules. RCCs presented lower levels of Fbln5. However, a fraction of RCCs presenting pulmonary metastasis revealed significantly higher levels of Fbln5 than those without metastasis, accompanied by immunopositivity of RCC cells and myofibroblast proliferation. Experiments revealed that RCC cell lines showed lower expression of fbln5 than its non-transformed counterpart NRK52E, but that fbln5 transfection to RCC cell lines changed neither proliferation nor migration/invasion. The data suggest that Fbln5 plays a role not only in the tissue repair and remodelling after renal tubular oxidative damage but also in RCC metastasis, presumably as a cytokine.

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.3109/10715762.2010.523702
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000287090300010&DestApp=WOS_CPL

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