論文

査読有り
2016年3月

Role of hemoglobin and transferrin in multi-wall carbon nanotube-induced mesothelial injury and carcinogenesis

CANCER SCIENCE
  • Yue Wang
  • ,
  • Yasumasa Okazaki
  • ,
  • Lei Shi
  • ,
  • Hiro Kohda
  • ,
  • Minoru Tanaka
  • ,
  • Kentaro Taki
  • ,
  • Tomoki Nishioka
  • ,
  • Tasuku Hirayama
  • ,
  • Hideko Nagasawa
  • ,
  • Yoriko Yamashita
  • ,
  • Shinya Toyokuni

107
3
開始ページ
250
終了ページ
257
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/cas.12865
出版者・発行元
WILEY-BLACKWELL

Multi-wall carbon nanotubes (MWCNT) are a form of flexible fibrous nanomaterial with high electrical and thermal conductivity. However, 50-nm MWCNT in diameter causes malignant mesothelioma (MM) in rodents and, thus, the International Agency of Research on Cancer has designated them as a possible human carcinogen. Little is known about the molecular mechanism through which MWCNT causes MM. To elucidate the carcinogenic mechanisms of MWCNT in mesothelial cells, we used a variety of lysates to comprehensively identify proteins specifically adsorbed on pristine MWCNT of different diameters (50 nm, NT50; 100 nm, NT100; 150 nm, NT150; and 15 nm/tangled, NTtngl) using mass spectrometry. We identified >400 proteins, which included hemoglobin, histone, transferrin and various proteins associated with oxidative stress, among which we selected hemoglobin and transferrin for coating MWCNT to further evaluate cytotoxicity, wound healing, intracellular catalytic ferrous iron and oxidative stress in rat peritoneal mesothelial cells (RPMC). Cytotoxicity to RPMC was observed with pristine NT50 but not with NTtngl. Coating NT50 with hemoglobin or transferrin significantly aggravated cytotoxicity to RPMC, with an increase in cellular catalytic ferrous iron and DNA damage also observed. Knockdown of transferrin receptor with ferristatin II decreased not only NT50 uptake but also cellular catalytic ferrous iron. Our results suggest that adsorption of hemoglobin and transferrin on the surface of NT50 play a role in causing mesothelial iron overload, contributing to oxidative damage and possibly subsequent carcinogenesis in mesothelial cells. Uptake of NT50 at least partially depends on transferrin receptor 1. Modifications of NT50 surface may decrease this human risk.

Web of Science ® 被引用回数 : 24

リンク情報
DOI
https://doi.org/10.1111/cas.12865
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26679080
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000373484300006&DestApp=WOS_CPL

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