論文

査読有り 国際誌
2019年2月4日

Generation of bat-derived influenza viruses and their reassortants.

Scientific reports
  • Masahiro Sato
  • Junki Maruyama
  • Tatsunari Kondoh
  • Naganori Nao
  • Hiroko Miyamoto
  • Yoshihiro Takadate
  • Wakako Furuyama
  • Masahiro Kajihara
  • Hirohito Ogawa
  • Rashid Manzoor
  • Reiko Yoshida
  • Manabu Igarashi
  • Ayato Takada
  • 全て表示

9
1
開始ページ
1158
終了ページ
1158
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-018-37830-x

Two novel influenza A virus-like genomes were detected in fruit bats in Central and South America. However, the biological properties of these bat-derived influenza viruses (BatIVs) are still largely unknown since infectious viral particles have never been isolated from the infected host species. In this study, a reverse genetics approach was used to generate infectious BatIV particles entirely from plasmids encoding full-length sequences in eight gene segments. We inoculated BatIV particles into various cell cultures including bat-derived cell lines and found that BatIVs infected particular bat-derived cells efficiently but not the other cell lines tested. Reassortant viruses between the two BatIVs were also successfully generated and their replication in the susceptible bat cell lines was confirmed. These findings suggest a limited host range and reassortment potential of BatIVs in nature, providing fundamental information for understanding of the ecology of BatIVs.

リンク情報
DOI
https://doi.org/10.1038/s41598-018-37830-x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30718752
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362294
ID情報
  • DOI : 10.1038/s41598-018-37830-x
  • PubMed ID : 30718752
  • PubMed Central 記事ID : PMC6362294

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