荒井一人 (Kazuhito Arai) - マイポータル

基本情報

学位: 博士（理学）(東京理科大学)

J-GLOBAL ID: 200901098577840537
researchmap会員ID: 6000005001

研究分野

ナノテク・材料 / 生物分子化学 /

学歴

MISC

Active and inactive state structures of unliganded Lactabacillus casei allosteric L-lactate dehydrogenase

Kazuhito Arai, Toshihiro Ishimitsu, Shinya Fushinobu, Hiroyuki Uchikoba, Hiroshi Matsuzawa, Hayao Taguchi

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 78(3) 681-694 2010年2月
Active and inactive state structures of unliganded Lactabacillus casei allosteric L-lactate dehydrogenase

Kazuhito Arai, Toshihiro Ishimitsu, Shinya Fushinobu, Hiroyuki Uchikoba, Hiroshi Matsuzawa, Hayao Taguchi

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 78(3) 681-694 2010年2月
A new family of D-2-hydroxyacid dehydrogenases that comprises D-mandelate dehydrogenases and 2-ketopantoate reductases

Yusuke Wada, Saho Iwai, Yusuke Tamura, Tomonori Ando, Takeshi Shinoda, Kazuhito Arai, Hayao Taguchi

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 72(4) 1087-1094 2008年4月
A new family of D-2-hydroxyacid dehydrogenases that comprises D-mandelate dehydrogenases and 2-ketopantoate reductases

Yusuke Wada, Saho Iwai, Yusuke Tamura, Tomonori Ando, Takeshi Shinoda, Kazuhito Arai, Hayao Taguchi

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 72(4) 1087-1094 2008年4月
A highly specific glyoxylate reductase derived from a formate dehydrogenase

Takeshi Shinoda, Kazuhito Arai, Hayao Taguchi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 355(3) 782-787 2007年4月
A highly specific glyoxylate reductase derived from a formate dehydrogenase

Takeshi Shinoda, Kazuhito Arai, Hayao Taguchi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 355(3) 782-787 2007年4月
Distinct conformation-mediated functions of an active site loop in the catalytic reactions of NAD-dependent D-lactate dehydrogenase and formate dehydrogenase

T Shinoda, K Arai, M Shigematsu-Iida, Y Ishikura, S Tanaka, T Yamada, MS Kimber, EF Pai, S Fushinobu, H Taguchi

JOURNAL OF BIOLOGICAL CHEMISTRY 280(17) 17068-17075 2005年4月
Distinct conformation-mediated functions of an active site loop in the catalytic reactions of NAD-dependent D-lactate dehydrogenase and formate dehydrogenase

T Shinoda, K Arai, M Shigematsu-Iida, Y Ishikura, S Tanaka, T Yamada, MS Kimber, EF Pai, S Fushinobu, H Taguchi

JOURNAL OF BIOLOGICAL CHEMISTRY 280(17) 17068-17075 2005年4月
Conversion of Lactobacillus pentosus D-lactate dehydrogenase to a D-hydroxyisocaproate dehydrogenase through a single amino acid replacement

C Tokuda, Y Ishikura, M Shigematsu, H Mutoh, S Tsuzuki, Y Nakahira, Y Tamura, T Shinoda, K Arai, O Takahashi …

JOURNAL OF BACTERIOLOGY 185(16) 5023-5026 2003年8月
Conversion of Lactobacillus pentosus D-lactate dehydrogenase to a D-hydroxyisocaproate dehydrogenase through a single amino acid replacement

C Tokuda, Y Ishikura, M Shigematsu, H Mutoh, S Tsuzuki, Y Nakahira, Y Tamura, T Shinoda, K Arai, O Takahashi …

JOURNAL OF BACTERIOLOGY 185(16) 5023-5026 2003年8月
Two forms of NAD-dependent D-mandelate dehydrogenase in Enterococcus faecalis IAM 10071

Y Tamura, A Ohkubo, S Iwai, Y Wada, T Shinoda, K Arai, S Mineki, M Iida, H Taguchi

APPLIED AND ENVIRONMENTAL MICROBIOLOGY 68(2) 947-951 2002年2月
Two forms of NAD-dependent D-mandelate dehydrogenase in Enterococcus faecalis IAM 10071

Y Tamura, A Ohkubo, S Iwai, Y Wada, T Shinoda, K Arai, S Mineki, M Iida, H Taguchi

APPLIED AND ENVIRONMENTAL MICROBIOLOGY 68(2) 947-951 2002年2月
An absolute requirement of fructose 1,6-bisphosphate for the Lactobacillus casei L-lactate dehydrogenase activity induced by a single amino acid substitution

K Arai, A Hishida, M Ishiyama, T Kamata, H Uchikoba, S Fushinobu, H Matsuzawa, H Taguchi

PROTEIN ENGINEERING 15(1) 35-41 2002年1月
An absolute requirement of fructose 1,6-bisphosphate for the Lactobacillus casei L-lactate dehydrogenase activity induced by a single amino acid substitution

K Arai, A Hishida, M Ishiyama, T Kamata, H Uchikoba, S Fushinobu, H Matsuzawa, H Taguchi

PROTEIN ENGINEERING 15(1) 35-41 2002年1月
Some Lactobacillus L-lactate dehydrogenases exhibit comparable catalytic activities for pyruvate and oxaloacetate

K Arai, T Kamata, H Uchikoba, S Fushinobu, H Matsuzawa, H Taguchi

JOURNAL OF BACTERIOLOGY 183(1) 397-400 2001年1月
Some Lactobacillus L-lactate dehydrogenases exhibit comparable catalytic activities for pyruvate and oxaloacetate

K Arai, T Kamata, H Uchikoba, S Fushinobu, H Matsuzawa, H Taguchi

JOURNAL OF BACTERIOLOGY 183(1) 397-400 2001年1月

講演・口頭発表等

乳酸菌Lactobacillus casei LDHのサブユニット間イオン結合ネットワーク導入によるアロステリック型から非アロステリック型LDHへの変換

日本蛋白質科学会 2009年
乳酸菌Lactobacillus casei アロステリックLDHのリガンドを結合していない活性型および不活性型構造

日本蛋白質科学会 2008年
乳酸菌Lactobacillus casei LDHアロステリック転移におけるサブユニット間連鎖運動

日本生化学会 2008年
Lactobacillus pentosus由来のD型乳酸脱水素酵素（D-LDH）の基質特異性改変

日本生化学会 2008年
高度好熱性細菌Thermus caldophilus由来アロステリックL型乳酸脱水素酵素のX線結晶構造解析

第7回日本蛋白質科学会年会 2007年

荒井一人

基本情報

研究分野

学歴

MISC

講演・口頭発表等

共同研究・競争的資金等の研究課題

メニュー