2018年5月9日
Facile determination of sphingolipids under alkali condition using metal-free column by LC-MS/MS
Analytical and Bioanalytical Chemistry
- ,
- ,
- 巻
- 410
- 号
- 20
- 開始ページ
- 1
- 終了ページ
- 11
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s00216-018-1116-5
- 出版者・発行元
- Springer Verlag
Extraction and analysis of sphingolipids from biological samples is a critical step in lipidomics, especially for minor species such as sphingoid bases and sphingosine-1-phosphate. Although several liquid chromatography-mass spectrometry methods enabling the determination of sphingolipid molecular species have been reported, they were limited in analytical sensitivity and reproducibility by causing significant peak tailing, especially by the presence of phosphate groups, and most of the extraction techniques are laborious and do not cover a broad range of sphingolipid metabolites. In this study, we developed a rapid single-phase extraction and highly sensitive analytical method for the detection and quantification of sphingolipids (including phosphates) comprehensively using liquid chromatography-triple quadruple mass spectrometry. After validating the reliability of the method, we analyzed the intestinal tissue sphingolipids of germ-free (GF) and specific pathogen-free (SPF) mice and found significantly higher levels of free sphingoid bases and sphingosine-1-phosphate in the GF condition as compared to the SPF condition. This method enables a rapid extraction and highly sensitive determination of sphingolipids comprehensively at low femtomolar ranges. [Figure not available: see fulltext.]
- リンク情報
- ID情報
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- DOI : 10.1007/s00216-018-1116-5
- ISSN : 1618-2650
- ISSN : 1618-2642
- SCOPUS ID : 85046680755