論文

査読有り
2019年3月

Application of novel sialoglyco-particulates enhances the detection sensitivity of equine influenza virus by real-time RT-PCR

ACS Applied Bio Materials
  • Ogata Makoto
  • Yamanaka Takashi
  • Koizumi Ami
  • Sakamoto Mao
  • Aita Rena
  • Endo Hiroyuki
  • Yachi Takehiro
  • Yamauchi Noriko
  • Otsubo Tadamune
  • Ikeda Kiyoshi
  • Kato Tatsuya
  • Park Enoch
  • Kono Hiroyuki
  • Nemoto Manabu
  • Hidari Kazuya
  • 全て表示

2
3
開始ページ
1255
終了ページ
1261
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/acsabm.8b00813

Sialoglyco-particulates carrying a N-glycolylneuraminyl-α-(2→3)-N-acetyllactosamine (Neu5Gcα2,3LacNAc) residue that displays a high level of affinity for equine influenza virus (EIV) were generated using sialoglycopolypeptide and hexyl-containing hybrid silica particulates. The particulates were spherical with a diameter of approximately 950 nm and found to have good dispersibility in aqueous solution. Interaction between the sialoglyco-particulates and EIV was investigated by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) of the EIV genome captured on the particulates. The number of EIV-specific genes detected by rRT-PCR on a nasal swab obtained from infected horses clearly increased when the sample was treated with sialoglyco-particulates. Our results show these novel sialoglyco-particulates can be used as a highly sensitive tool for detecting low levels of EIV that were previously undetectable in the early or late stage of infection.

リンク情報
DOI
https://doi.org/10.1021/acsabm.8b00813
URL
https://pubs.acs.org.ccindex.cn/doi/10.1021/acsabm.8b00813
ID情報
  • DOI : 10.1021/acsabm.8b00813
  • ISSN : 2576-6422
  • eISSN : 2576-6422

エクスポート
BibTeX RIS