論文

査読有り
2009年4月

Detection of diatom in formalin-fixed organs by enzymatic digestion

Legal Medicine
  • Toshiaki Takeichi
  • ,
  • Osamu Kitamura

11
1
開始ページ
S348
終了ページ
S349
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.legalmed.2009.01.060

The detection of diatoms has been proposed to be useful in the diagnosis of drowning. Enzymatic digestion of unfixed lungs and other organs is widely used to detect diatoms. In the present study, we examined the application of enzymatic digestion for diatom detection in formalin-fixed organs obtained at autopsy. Furthermore, we assessed the effect of hydrogen peroxide on contamination of the lung specimen with foreign bodies inhaled in the course of drowning or as a result of smoking or air pollution. Unfixed lung was digested with proteinase K for 2 d and centrifuged
the supernatant was then removed. On the other hand, formalin-fixed lung samples were boiled in Tris-HCl buffer containing sodium dodecyl sulfate (SDS) and then subjected to enzymatic digestion. A portion of formalin-fixed or unfixed samples digested with proteinase K was incubated with hydrogen peroxide at 80 °C and mounted on a slide, while the remaining portion was processed without incubation. Samples incubated with hydrogen peroxide showed clearer image of diatoms than those not incubated with hydrogen peroxide
this is because the inhaled foreign bodies were discolored in the former samples. Therefore, incubation of digested samples with hydrogen peroxide would be helpful for quantitative and qualitative analyses of diatoms. © 2009 Elsevier Ireland Ltd. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.legalmed.2009.01.060
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19261514
ID情報
  • DOI : 10.1016/j.legalmed.2009.01.060
  • ISSN : 1344-6223
  • PubMed ID : 19261514
  • SCOPUS ID : 67349123687

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