2020年
Base editors for simultaneous introduction of C-to-T and A-to-G mutations
Nature Biotechnology
- 巻
- 38
- 号
- 7
- 開始ページ
- 901
- 終了ページ
- 901
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1038/s41587-020-0509-0
- 出版者・発行元
- Nature Biotechnology
© 2020, The Author(s), under exclusive licence to Springer Nature America, Inc. We describe base editors that combine both cytosine and adenine base-editing functions. A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase TadA and a Cas9 nickase (Target-ACEmax) showed a high median simultaneous C-to-T and A-to-G editing activity at 47 genomic targets. On-target as well as DNA and RNA off-target activities of Target-ACEmax were similar to those of existing single-function base editors.
- リンク情報
- ID情報
-
- DOI : 10.1038/s41587-020-0509-0
- ISSN : 1087-0156
- PubMed ID : 32504047