2019年11月21日
Ligand Binding-Induced Structural Changes in the M2 Muscarinic Acetylcholine Receptor Revealed by Vibrational Spectroscopy.
The journal of physical chemistry letters
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- 巻
- 10
- 号
- 22
- 開始ページ
- 7270
- 終了ページ
- 7276
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/acs.jpclett.9b02942
M2 muscarinic acetylcholine receptor (M2R) is a prototypical G protein-coupled receptor (GPCR) that responds to acetylcholine and mediates various cellular responses in the nervous system. Here, we used attenuated total reflection-Fourier transform infrared spectroscopy analyses on M2R reconstituted in a lipid membrane to understand the molecular mechanism behind the ligand binding-induced conformational changes. Upon agonist binding, M2R shows large spectral change of the amide-I band corresponding to backbone C═O stretch, which likely connects with the receptor activation in the lipid environment. These results pave the way to probe effects of different ligand binding on GPCRs using vibrational spectroscopy.
- リンク情報
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- DOI
- https://doi.org/10.1021/acs.jpclett.9b02942
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/31692352
- URL
- http://europepmc.org/abstract/med/31692352
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85075168229&origin=inward
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85075168229&origin=inward
- ID情報
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- DOI : 10.1021/acs.jpclett.9b02942
- eISSN : 1948-7185
- ORCIDのPut Code : 88990271
- PubMed ID : 31692352
- SCOPUS ID : 85075168229