2015年9月
Reduction of distortion in photothermal microscopy and its application to the high-resolution three-dimensional imaging of nonfluorescent tissues
BIOMEDICAL OPTICS EXPRESS
- ,
- ,
- 巻
- 6
- 号
- 9
- 開始ページ
- 3217
- 終了ページ
- 3224
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1364/BOE.6.003217
- 出版者・発行元
- OPTICAL SOC AMER
A scheme for reducing image distortion in photothermal microscopy is presented. In photothermal microscopy, the signal shape exhibits twin peaks corresponding to the focusing or defocusing of the probe beam when a sample is scanned in the axial direction. This causes a distortion when imaging a structured sample in the axial plane. Here, we demonstrate that image distortion caused by the twin peaks is effectively suppressed by providing a small offset between two the focal planes of the pump and the probe beams. Experimental results demonstrate improvement in resolution, especially in the axial direction, over conventional optical microscopy-even with the focal offset. When a dry objective lens with a numerical aperture of 0.95 is used, the full width at half the maximum of the axial point spread function is 0.6 mu m, which is 50% (62%) smaller than the focal spot sizes of the pump (probe) beam. Herein, we present high-resolution three-dimensional imaging of thick biological tissues based on the present scheme. (C) 2015 Optical Society of America
- リンク情報
- ID情報
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- DOI : 10.1364/BOE.6.003217
- ISSN : 2156-7085
- Web of Science ID : WOS:000360888400009