2015年5月
Sub-diffraction-limit imaging using mode multiplexing
OPTICS COMMUNICATIONS
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- 巻
- 343
- 号
- 開始ページ
- 28
- 終了ページ
- 33
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.optcom.2014.12.072
- 出版者・発行元
- ELSEVIER SCIENCE BV
Pixel-by-pixel processed fluorescence difference microscopy is experimentally demonstrated by multiplexing excitation laser beams with Gaussian and donut spot shapes and then demultiplexing the fluorescent signals using lock-in amplifiers. With this scheme, a fixed sample of fluorescent spheres and a slice of mouse brain tissue are imaged with resolutions that exceed the diffraction limit. Compared to previously reported subtraction imaging techniques, this pixel-by-pixel scan can be applied to improve the resolution of a moving sample without introducing subtraction errors. The synchronized signal detection feature makes this method extendible to various applications. (C) 2014 Elsevier B.V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.optcom.2014.12.072
- ISSN : 0030-4018
- eISSN : 1873-0310
- Web of Science ID : WOS:000352045400005