論文

査読有り
2013年4月

Fiber-substituted Conditionally Replicating Adenovirus Ad5F35 Induces Oncolysis of Human Bladder Cancer Cells in In Vitro Analysis

UROLOGY
  • Akinobu Gotoh
  • ,
  • Hisao Nagaya
  • ,
  • Takeshi Kanno
  • ,
  • Masatoshi Tagawa
  • ,
  • Tomoyuki Nishizaki

81
4
開始ページ
920.e7
終了ページ
11
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.urology.2012.12.023
出版者・発行元
ELSEVIER SCIENCE INC

OBJECTIVE To assess the oncolytic effect of fiber-substituted conditionally replicating adenovirus type 5 (Ad5) F35 vector on human bladder cancer cell lines such as 253J, 5637, KK-47, T24, TCCSUP, and UMUC-3 cells.
MATERIALS AND METHODS Ad5F35 and Ad5 conditionally replicating adenovirus vectors containing the E1 gene controlled by the human midkine promoter (Ad5F35/MKp-E1 and Ad5/MKp-E1, respectively) were constructed. Reverse transcriptase-polymerase chain reaction and cell viability assay were performed in cells transfected with Ad5F35/MKp-E1 or Ad5/MKp-E1.
RESULTS Of the bladder cancer cells used, considerably lower expression of mRNA for Coxsackie and adenovirus receptor, an Ad5 receptor, was found with T24 and TCCSUP cells. However, the mRNA for CD46, an Ad35 receptor, was abundantly expressed in all the cell types. Ad5F35/MKp-E1 induced oncolysis in a plaque formation unit-dependent manner for all the bladder cancer cells used, with greater efficacy than Ad5/MKp-E1 for T24, TCCSUP, and 253J cells.
CONCLUSION The results of the present study have shown that Ad5F35/MKp-E1 is more useful for the gene therapy of bladder cancer than Ad5/MKp-E1 is for some cell lines. UROLOGY 81: 920.e7-920.e11, 2013. (C) 2013 Elsevier Inc.

Web of Science ® 被引用回数 : 6

リンク情報
DOI
https://doi.org/10.1016/j.urology.2012.12.023
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23394881
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000317116300070&DestApp=WOS_CPL

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